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使用单克隆抗体(MAb)的免疫测试系统是多种研究必不可少的工具,但很少用于分子量较小的天然活性成分的检测。柴胡皂苷a(SSa)是柴胡的主要成分,作者采用抗一SSa MAb,开发了酶联免疫吸附法(ELISA)用于SSa的定量分析。
Immunoassay systems using monoclonal antibodies (MAbs) are indispensable tools for a variety of studies, but are rarely used for the detection of naturally occurring active ingredients with small molecular weights. Saikosaponin a (SSa) is the main component of Bupleurum chinense. The authors developed an enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of SSa using an anti-SSa MAb.