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采用免疫组化法、酶联免疫法检测20只博来霉素诱导硬皮病小鼠动物模型皮肤、血浆及肺组织TGF-β1、ET-1、Ang Ⅱ的表达水平,并以20只正常小鼠作为对照。①免疫组化法检测博来霉素诱导硬皮病小鼠皮损中TGF-β1表达程度高于对照,纤维化肺组织中表达程度也高于对照;皮损及纤维化肺组织中ET-1表达程度也高于对照;但Ang Ⅱ在皮损及纤维化肺组织中表达与对照无明显差异。②ELISA法检测博来霉素诱导硬皮病小鼠血浆及肺组织中TGF-β1表达上调;ET-1在血浆中表达水平两组无显著差异,在肺组织中表达水平明显高于对照;Ang Ⅱ在血浆及纤维化肺组织中表达均明显高于对照。提示博来霉素诱导硬皮病小鼠发病与上述细胞因子异常表达有关。
Immunohistochemistry and enzyme-linked immunosorbent assay were used to detect the expression of TGF-β1, ET-1 and Ang Ⅱ in 20 bleomycin-induced scleroderma mouse models of skin, plasma and lung tissue. Mice served as controls. ① Immunohistochemical detection of bleomycin-induced skin lesions in mice with scleroderma TGF-β1 expression was higher than the control, fibrosis in lung tissue expression was higher than the control; lesions and fibrosis in the lung tissue ET- 1 expression was also higher than the control; but Ang Ⅱ in the lesion and fibrosis in lung tissue expression and no significant difference. ② The ELISA method was used to detect the expression of TGF-β1 in bleomycin-induced scleroderma mice plasma and lung tissue. The expression level of ET-1 in plasma was no significant difference between the two groups, and the level of ET-1 in lung tissue was significantly higher than that of the control Ⅱ in plasma and fibrosis of lung tissue were significantly higher than the control. Prompted bleomycin-induced scleroderma mouse pathogenesis and abnormal expression of the above cytokines.