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目的探讨中国徐州地区汉族人群内皮脂肪酶(endothelial lipase,LIPG)基因启动子rs9958947C>T单核苷酸多态性与腔隙性脑梗死的相关性。方法采用病例-对照研究的方法,选取189例腔隙性脑梗死患者为病例组,另选202例健康体检者为对照组,采用聚合酶链反应和限性片段长度多态性方法,检测2组LIPG启动子rs9958947单核苷酸多态性。结果病例组中T等位基因频率(51.59%)和CT+TT基因型频率(78.31%)明显高于对照组(分别为43.56%和66.83%);2种基因频率组间比较分别为x~2=5.041,P=0.025和x2=6.431,P=0.011,差异具有统计学意义;Logistic回归分析显示,T等位基因、男性、吸烟、高血压、高血脂、糖尿病是徐州地区汉族人群腔隙性脑梗死的独立危险因素。结论 LIPG基因启动子rs9958947单核苷酸多态性与腔隙性脑梗死发病具有相关性。
Objective To investigate the association of SNP rs9958947C> T polymorphism in LIPG gene with lacunar infarction in Han nationality in Xuzhou, China. Methods A case-control study was conducted in 189 cases of lacunar infarction patients and 202 cases of healthy volunteers as control group. Polymerase chain reaction and restriction fragment length polymorphism Group LIPG promoter rs9958947 single nucleotide polymorphism. Results The frequencies of T allele (51.59%) and CT + TT genotype (78.31%) were significantly higher in the case group than in the control group (43.56% and 66.83% respectively). The frequencies of the two genotypes were x ~ 2 = 5.041, P = 0.025 and x2 = 6.431, P = 0.011, the difference was statistically significant; Logistic regression analysis showed that the T allele, male, smoking, hypertension, hyperlipidemia and diabetes were the lacunar of Han nationality in Xuzhou Independent risk factors for cerebral infarction. Conclusion The single nucleotide polymorphism of rs9958947 in LIPG gene is correlated with the pathogenesis of lacunar infarction.