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目的:探讨羊卵巢组织玻璃化冷冻复苏后异种移植的卵泡生长发育情况。方法:取绵羊的卵巢,将卵巢皮质切块,应用玻璃化快速冷冻法保存。羊卵巢组织片复苏后在裸鼠的颈部皮下移植,每只裸鼠移植2块组织,于移植1个月后获取移植物,HE染色观察存活卵泡的情况。结果:共移植组织16块,取材时肉眼可见其中一块组织纤维化萎缩,镜下见此块组织纤维化,移植组织存活率87.5%(14/16)。HE染色观察每只移植小鼠的2块移植物中基本只有1块有可视卵泡。1只鼠未见可视卵泡;1只鼠见1个不典型原始卵泡;1只鼠有2个不典型原始卵泡;余下5只鼠的移植卵巢组织中均可见多个典型的存活卵泡。结论:羊卵巢组织经冷冻复苏后能够成功移植于裸鼠的颈部皮下,大部分卵泡处于良好的存活状态。
Objective: To investigate the growth and development of ovarian follicles after ovarian tissue vitrification. Methods: Sheep ovary, the ovarian cortex cut, the use of glassy rapid freezing method. Sheep ovary tissue pieces were transplanted subcutaneously in the nude mice neck after transplanted. Two nude mice were transplanted into the nude mice. The grafts were obtained 1 month after transplantation and the survival follicles were observed by HE staining. Results: A total of 16 tissues were transplanted. One piece of tissue atrophy was observed with the naked eye. The tissue fibrosis was seen microscopically. The survival rate of the transplanted tissue was 87.5% (14/16). Only two of the two grafts in each transplanted mouse were visualized as visible follicles by HE staining. 1 mouse showed no visible follicles; 1 mouse showed 1 atypical primordial follicles; 1 mouse had 2 atypical primordial follicles; and the remaining 5 mice showed multiple typical ovarian follicles. Conclusion: Sheep ovary tissue can be successfully transplanted subcutaneously into the neck of nude mice after cryopreservation, and most follicles are in good survival state.