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目的:观察不同灸法中穴位、施灸壮数与施灸时间对皮肤表里温度的影响,为临床灸量标准化制定提供参考.方法:将42只大耳白兔按随机数字表法分为6组,即直接灸1壮组、直接灸2壮组、直接灸3壮组、隔药饼灸1壮组、隔药饼灸2壮组和隔药饼灸3壮组,每组7只.各组均取神阙、肾俞和足三里,但施灸方法、施灸壮数及施灸时间不同.各组均隔日施灸1次,共灸5次.治疗过程中用热电偶探针和温度记录仪记录穴位皮肤实时温度以及相关时间节点温度,以观察艾灸穴位皮肤表里温度差异的实时变化,并进行分析处理.结果:隔药饼灸在表面最高温度为(49.20±0.52)℃时,最佳易壮温度为(46.38±0.51)℃;穴位×壮数×时间和穴位×灸法×时间的多因素比较显示时间×穴位、时间×灸法及壮数×穴位有交互作用(均P<0.05).相同穴位不同壮数的皮肤温度差比较,神阙在实验第1天和第5天,肾俞在实验第3天和第7天,足三里在实验第1天及第9天有统计学差异(均P<0.05).相同穴位不同灸法的皮肤温度比较,除神阙艾灸前及肾俞艾灸前和第5天外,其余均有统计学差异(均P<0.05);足三里仅在第5天及第7天有统计学差异(均P<0.05).不同穴位在施灸1壮、2壮及3壮后的皮肤温度差均有统计学差异(均P<0.05);不同穴位直接灸与隔药饼灸的结果均存在统计学差异(均P<0.05).结论:相同规格下隔药饼灸有确定的易壮温度.不同穴位在相同最高温的情况下皮肤表里温度差因施灸壮数和灸法的不同而表现出明显差异,其中肾俞皮肤温度差最高,神阙其次,足三里最低.在进行灸疗量化指标确定时应考虑穴位因素的影响.“,”Objective: To observe the effects of acupoints, cone numbers and durations of moxibustion with different moxibustion methods on skin surface and inside temperature, and to provide references for the clinical standardization of moxibustion amount. Methods: The 42 big-ear white rabbits were divided into 6 groups according to the random number table method, a 1-cone direct moxibustion group, a 2-cone direct moxibustion group, a 3-cone direct moxibustion group, a 1-cone herbal cake-partitioned moxibustion group, a 2-cone herbal cake-partitioned moxibustion group, and a 3-cone herbal cake-partitioned moxibustion group, with 7 rabbits in each group. Shenque (CV 8), Shenshu (BL 23) and Zusanli (ST 36) were used in each group, but the moxibustion methods, cone numbers and durations of moxibustion were different. Rabbits in each group received moxibustion once every other day for 5 times in total. During the intervention, a thermoelectricity coupled probe and a temperature recorder were used to record the real-time acupoint skin temperature and the temperature at different time points, so as to observe, analyze and process the real-time changes in the temperature difference between the surface and inside of acupoint skin. Results: For herbal cake-partitioned moxibustion, the best temperature for cone changing was (46.38±0.51) ℃ when the highest surface temperature was (49.20±0.52) ℃; the multi-factor comparison of acupoint × cone number × time and acupoint × moxibustion method × time showed that time × acupoint, time × moxibustion method and cone number × acupoint had interactive effects (all P<0.05). Comparing skin temperature differences between different cone numbers at the same acupoint, Shenque (CV 8) on the 1st and the 5th days, Shenshu (BL 23) on the 3rd and the 7th days, Zusanli (ST 36) on the 1st and the 9th days of experiment showed statistically significant differences (all P<0.05). The skin temperature comparison of different moxibustion methods at the same acupoint all had statistical differences (all P<0.05), except for Shenque (CV 8) before moxibustion, Shenshu (BL 23) before moxibustion and on the 5th day; Zusanli (ST 36) only showed statistical differences on the 5th and 7th days (both P<0.05). The skin temperature differences of different acupoints after moxibustion in the 1-cone, 2-cone and 3-cone groups were statistically different (all P<0.05); direct moxibustion and herbal cake-partitioned moxibustion at different acupoints were all statistically different (all P<0.05). Conclusion: Cone changing temperature under the same specifications of herbal cake-partitioned moxibustion was confirmed. Temperature difference between surface and inside of different acupoint skin at the same maximum temperature was significantly different due to the cone numbers and moxibustion methods, which showed the highest at Shenshu (BL 23), the second at Shenque (CV 8), and the lowest at Zusanli (ST 36). The influence of acupoint factor should be considered to determine the quantitative indicators of moxibustion.