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目的:研究不同干燥方法对马甲子鲜叶中抗肿瘤活性成分的影响,并优化其提取工艺参数。方法:采用HPLCELSD测定马甲子素和白桦脂酸的含量,流动相乙腈(A)-0.1%甲酸溶液(B)梯度洗脱(0~10 min,55%A;10~35 min,55%~100%A),检测波长320 nm。考察不同干燥方法对马甲子药材中有效成分含量的影响;以马甲子素和白桦脂酸提取量的综合评分为指标,通过正交试验考察乙醇体积分数、乙醇用量和提取时间对有效成分提取工艺的影响。结果:冷冻干燥和阴干法处理时,白桦脂酸和马甲子素的保存效果较好。最佳提取工艺为加4倍量95%乙醇冷浸提取2次,每次48 h;此工艺下测得冷冻干燥样品中白桦脂酸和马甲子素平均质量分数分别为4.61,0.077 4 mg·g-1。结论:马甲子的干燥方法适宜采用冷冻干燥法,但考虑大生产成本,建议选择阴干法。优化的提取工艺稳定合理、有效成分提取率高,为马甲子药材资源的开发利用提供参考。
Objective: To study the effects of different drying methods on the antitumor active ingredients in the fresh leaves of Paeonia lactiflora and to optimize its extraction process parameters. Methods: The contents of pachyrin and betulinic acid were determined by HPLCELSD. The mobile phase was eluted with a gradient of acetonitrile (A) -0.1% formic acid (B) (0-10 min, 55% A; 10-35 min, 100% A), detection wavelength 320 nm. The effects of different drying methods on the content of active ingredients in the seeds of Paeonia lactiflora Pall. Were studied. Taking the comprehensive score of the extractive amounts of pockeys and betulinic acid as indexes, the orthogonal test was used to study the effects of ethanol volume fraction, ethanol dosage and extraction time on the extraction of active ingredients Impact. Results: The preservative effect of betulinic acid and calico is better than that of freeze-dried and dry-processed. The best extraction process was cold extraction of 4 times amount of 95% ethanol twice for 48 hours. The average contents of betulinic acid and pachymanin in freeze-dried samples were 4.61,0.077 4 mg · g-1. Conclusion: The suitable method for drying the kojicar is freeze-drying method. However, considering the large-scale production cost, it is recommended to choose the method of dry-shade. The optimized extraction process is stable and reasonable, the extraction rate of active ingredients is high, which provides a reference for the development and utilization of the resources of the Chinese medicine.