论文部分内容阅读
目的了解福建厦门、广东深圳及广州的鼠形动物脑组织标本寨卡病毒的携带情况,分析鼠形动物作为人类寨卡病毒病潜在传染源的可能性。方法于2013年1月—2016年6月在厦门、深圳及广州(白云区、越秀区)部分居民区、城市公园、医院、农贸市场、学校捕获鼠形动物,无菌采集其脑组织样本,用逆转录聚合酶链反应(RT-PCR)方法检测寨卡病毒核酸。结果本次调查共捕获鼠形动物1 092只2目2科3属7种,家鼠以褐家鼠为优势鼠种(59.84%),野鼠以黄毛鼠为优势鼠种(98.02%);共获1 092份脑组织标本,RT-PCR方法在采集的脑组织标本中均未检测出寨卡病毒核酸。结论厦门、深圳及广州鼠形动物作为寨卡病毒病传染源的可能性小。
Objective To understand the carriage of Zika virus in mouse brain samples of Xiamen, Guangdong, Shenzhen and Guangzhou in Guangdong Province, and to analyze the possibility of mouse as a potential infection source of human Zika virus disease. Methods From January 2013 to June 2016, murine animals were captured in some residential areas, urban parks, hospitals, farmer’s markets and schools in Xiamen, Shenzhen and Guangzhou (Baiyun District and Yuexiu District), and their brain samples were aseptically collected. Zika virus nucleic acid was detected by reverse transcription polymerase chain reaction (RT-PCR). Results A total of 1 092 species of 2 species, 2 families, 3 genera and 7 species were captured in this survey. Rattus norvegicus was the dominant species in domestic mice (59.84%), while rodent species were dominant species in rodents (98.02%). A total of 1 092 brain tissue samples were obtained. No Zika virus nucleic acid was detected in the collected brain tissue samples by RT-PCR. Conclusions Xiamen, Shenzhen and Guangzhou mice are less likely to be the source of Zika virus infection.