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目的探讨凋亡相关基因survivin、bcl-2及caspase-3在胆囊癌中的表达及其在胆囊癌发生、发展的可能作用及相互关系。方法应用免疫组织化学SABC法,检测39例胆囊癌组织、15例胆囊腺瘤组织和12例慢性胆囊炎组织中survivin、bcl-2及caspase-3表达情况,分析其与胆囊癌临床病理的关系,并探讨survivin、bcl-2及caspase-3表达在胆囊癌中的相关性。结果survivin在胆囊癌中的阳性表达率为71.8%,而在胆囊腺瘤及慢性胆囊炎中均无表达;bcl-2在胆囊癌中的表达阳性率为71.8%,而在胆囊腺瘤及慢性胆囊炎中均无表达;bcl-2在胆囊癌中的表达阳性率为38.5%,在胆囊腺瘤中为93.3%,在慢性胆囊炎中为8.3%,胆囊腺瘤bcl-2表达明显高于胆囊癌(P<0.01),胆囊癌bcl-2表达明显高于慢性胆囊炎组织(P<0.05)。caspase-3在胆囊癌中的表达阳性率为43.6%,胆囊腺瘤及慢性胆囊炎中caspase-3表达率均为100%。survivin表达与患者的临床病理无关。bcl-2及caspase-3的表达与胆囊癌患者的性别、年龄、肿瘤的大小无关,而阳性率在组织分化程度、不同Nevin分期组间差异有显著性(P<0.05)。survivin与bcl-2及caspase-3表达没有相关性(P>0.05),bcl-2与caspase-3表达具有良好的相关性(P<0.05)。结论survivin和bcl-2在胆囊癌中有较高表达,而caspase-3在胆囊癌中的表达下降;bcl-2与caspase-3可反映胆囊癌的某些临床病理特点;survivin、bcl-2及caspase-3共同调节胆囊癌细胞凋亡,进而影响胆囊癌的发生、发展。
Objective To investigate the expression of survivin, bcl-2 and caspase-3 in gallbladder carcinomas and its possible role in gallbladder carcinogenesis and its relationship with each other. Methods The expressions of survivin, bcl-2 and caspase-3 in 39 cases of gallbladder carcinoma, 15 cases of gallbladder adenoma and 12 cases of chronic cholecystitis were detected by immunohistochemical SABC method. The correlation between the expression of survivin, bcl-2 and caspase-3 was analyzed. , And to explore the correlation between the expression of survivin, bcl-2 and caspase-3 in gallbladder carcinomas. Results The positive rate of survivin in gallbladder carcinoma was 71.8%, but not in gallbladder adenoma and chronic cholecystitis. The positive rate of bcl-2 in gallbladder carcinoma was 71.8% The expression of bcl-2 in gallbladder carcinoma was 38.5%, 93.3% in gallbladder adenoma and 8.3% in chronic cholecystitis, the expression of bcl-2 in gallbladder adenoma was significantly higher than that in gallbladder adenoma The expression of bcl-2 in gallbladder carcinoma was significantly higher than that in chronic cholecystitis (P <0.05). The positive rate of caspase-3 expression in gallbladder carcinoma was 43.6%, while the expression rate of caspase-3 in gallbladder adenoma and chronic cholecystitis was 100%. Survivin expression is not related to the clinical pathology of patients. The expression of bcl-2 and caspase-3 was not related to the gender, age and tumor size of patients with gallbladder carcinoma, but the positive rate was significantly different between the different stages of tissue differentiation and nevin staging (P <0.05). Survivin had no correlation with the expression of bcl-2 and caspase-3 (P> 0.05). There was a good correlation between the expression of bcl-2 and caspase-3 (P <0.05). Conclusions Survivin and bcl-2 are highly expressed in gallbladder carcinomas, while caspase-3 is down-regulated in gallbladder carcinomas. Both bcl-2 and caspase-3 may reflect some clinicopathological characteristics of gallbladder carcinomas. Survivin and bcl- And caspase-3 co-regulate gallbladder cancer cell apoptosis, thereby affecting the occurrence and development of gallbladder cancer.