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本文用乙酰葡萄糖异硫氰酸酯(GITC)作柱前手性衍生化试剂,反相高效液相色谱法拆分氨基酸立体异构体。研究了不同构型氨基酸与GITC反应后所生成的衍生物的紫外吸收响应特点。探讨了16种氨基酸GITC衍生物的色谱分离情况。
Acetylglucosamine isothiocyanate (GITC) was used as pre-column chiral derivatization reagent to resolve the amino acid stereoisomers by RP-HPLC. The UV absorption response of the derivatives formed after the reaction of different amino acids with GITC was studied. The chromatographic separation of 16 amino acid GITC derivatives was investigated.