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用弓形虫可溶性抗原免疫ＢＡＬＢ／ｃ 小鼠，取脾细胞，与Ｓｐ２／０ 细胞融合，获得３ 株能稳定分泌抗弓形虫单克隆抗体的细胞。细胞培养上清液和腹水中的单克隆抗体效价分别为１∶１６ ～２５６ 和１０ － ６ ～１０ － ７ 。单抗类型均为ＩｇＭ。杂交瘤细胞经液氮冻存１２ 个月后，复苏生长良好，分泌活性稳定。在ＭｃＡｂ－ Ｄｏｔ－ ＥＬＩＳＡ 实验中，所能测出可溶性抗原的最小浓度为１０ ｎｇ／ ｍＬ
BALB / c mice were immunized with soluble antigen of Toxoplasma gondii and splenocytes were obtained and fused with Sp2 / 0 cells to obtain three cells that can stably secrete monoclonal antibody against Toxoplasma gondii. Monoclonal antibody titers in cell culture supernatants and ascites were 1:16 to 256 and 10 - 6 to 10 - 7, respectively. Monoclonal antibody types are IgM. After hybridoma cells were frozen in liquid nitrogen for 12 months, the resuscitated cells grew well and their secretion activity was stable. In McAb-Dot-ELISA experiments, the minimum detectable concentration of soluble antigen was 10 ng / mL