论文部分内容阅读
目的 探讨自然杀伤T细胞 (NKT)在Graves’病发病中的作用。方法 用表达有人TSH受体的细胞免疫NKT细胞缺如小鼠和野生型BLAB/c小鼠 ,每两周一次共 6次。在最后一次免疫结束后两周 ,处死小鼠 ,检测血清甲状腺素水平 ,TSH受体抗体及甲状腺病理改变。结果 免疫后的NKT细胞缺如小鼠 ,血清TT4 和TRAb水平轻度升高 ,并且与对照组比较 ,有显著性差异 ,但是在经免疫的NKT细胞缺如小鼠和野生型小鼠之间 ,血清平均TT4 水平和TRAb水平相似 ,且TSAb与TSBAb水平变化两组之间无显著性差异。免疫后的NKT细胞缺如小鼠与野生小鼠甲状腺病理改变相似 ,但是与未经免疫的小鼠比较 ,表现为甲状腺滤泡细胞轻度增生。结论 NKT细胞可能不参与Graves’病的发生
Objective To investigate the role of natural killer T cells (NKT) in the pathogenesis of Graves’ disease. Methods NKT cells immunized with human TSH receptor-expressing cells were depleted of mouse and wild type BLAB / c mice a total of 6 times every two weeks. Two weeks after the last immunization, mice were sacrificed and serum thyroxine levels, TSH receptor antibodies and thyroid pathological changes were examined. Results After immunization, NKT cells were absent in mice, serum TT4 and TRAb levels were slightly elevated, and compared with the control group, there was a significant difference, but NKT cells in immune deficient mice and wild type mice , Serum mean TT4 levels and TRAb levels were similar, and TSAb and TSBAb levels between the two groups no significant difference. Immune NKT cell deficient mice showed similar pathological changes in the thyroid gland in wild mice, but showed mild hyperplasia of thyroid follicular cells compared with non-immunized mice. Conclusion NKT cells may not participate in the development of Graves’ disease