Objective: To clone tumor suppressor gene Fhit from normal human appendix’s tissue and construct its cloning vector. Methods: Extract human total RNA with TRIzol reagent and amplification the Fhit gene with reverse transcription-PCR (RT-PCR), then clone Fhit into a pGEM-T easy cloning vector, lastly identify the pGEM-Fhit vector with polymerase chain reaction (PCR), endonuclease digestion and DNA sequencing. Results: Fhit complementary DNA (cDNA) was cloned into pGEM-T vector, and DNA sequencing was the same as reported. Conclusions: The cloning vector pGEM-Fhit was constructed successfully and builds up a good foundation going deep into the study of Fhit. Method: Extract human total RNA with TRIzol reagent and amplification the Fhit gene with reverse transcription-PCR (RT-PCR), then clone Fhit into a pGEM -T easy cloning vector, lastly identified the pGEM-Fhit vector with polymerase chain reaction (PCR), endonuclease digestion and DNA sequencing. Results: Fhit complementary DNA (cDNA) was cloned into pGEM-T vector, and DNA sequencing was the same as Reported. Conclusions: The cloning vector pGEM-Fhit was constructed successfully and builds up a good foundation going deep into the study of Fhit.