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目的:研究N-乙酰半胱氨酸(NAC)对白细胞介素18(IL-18)诱导血管平滑肌细胞(VSMC)中肿瘤坏死因子α(TNF-α)及白细胞介素6(IL-6)表达的影响。方法:培养的VSMC细胞,加入或不加入NAC(5mmol/L)处理1h后,再以不同浓度的IL-18刺激不同时间,通过ELISA测定培养上清IL-6及TNF-α蛋白质水平,加入IL-18(100μg/L)6h后RT-PCR分析细胞中相应的mRNA水平。同时Westernblot分析NAC对IL-18激活的NF-κB的影响。结果:通过IL-18刺激的VSMC培养上清中的IL-6及TNF-α水平及细胞中的mRNA水平显著升高(P<0.01),且具有时间和剂量依赖关系(P<0.01)。NAC(5mmol/L)显著抑制IL-18诱导的IL-6及TNF-α的mRNA和蛋白质水平表达(P<0.01)。Westernblot分析显示NAC能够抑制IL-18对NF-κB的激活作用。结论:NAC体外可以抑制IL-18在SMC中诱导的IL-6及TNF-α表达。
Objective: To investigate the effect of NAC on the expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in vascular smooth muscle cells (VSMC) induced by interleukin- The impact of expression. Methods: VSMC cells were cultured with or without NAC (5mmol / L) for 1h, then stimulated with different concentrations of IL-18 for different time. The protein levels of IL-6 and TNF- IL-18 (100μg / L) 6h RT-PCR analysis of the corresponding mRNA levels in the cells. At the same time Western blot analysis of NAC IL-18-activated NF-κB. Results: The levels of IL-6 and TNF-α in supernatant of VSMC stimulated by IL-18 and the level of mRNA in cells were significantly increased (P <0.01) in a dose-dependent and time-dependent manner (P <0.01). NAC (5mmol / L) significantly inhibited IL-18-induced IL-6 and TNF-α mRNA and protein expression (P <0.01). Western blot analysis showed that NAC can inhibit the activation of NF-κB by IL-18. Conclusion: NAC can inhibit IL-18 induced IL-6 and TNF-α expression in SMC in vitro.