[Objectives] To observe the pharmaceutical effect of Zijin Huadu suppository on treatment of cervicitis induced by virus,bacteria and phenol in vivo and in vitro,and provide experimental basis for evaluating its therapeutic effects for cervicitis. [Methods]Real-time RT-PCR and CPE methods were used to observe the inhibition of Zijin Huadu suppository on virus,and turbidimetry was used to observe bacteriostatic action; HE pathological section was used to observe cervicitis of rat,and enzyme-linked immunosorbent assay was used to detect virus’ s expression in cervical tissue.[Results]In cervicitis rat model infected by HPV16,Zijin Huadu suppositories at the doses of 1. 2,0. 6 and0. 3 g/( kg·d) had significant inhibitory effect on HPV16 expression in cervical tissues,and the doses of 1. 2 and 0. 6 g/( kg·d) had significantly inhibitory effect on pathological changes of cervical tissues. In cervicities rat models infected mixedly by Escherichia coli,Neisseria gonorrhoeae and Staphylococcus aureus,Zijin Huadu suppositories at the doses of 1. 2 and 0. 6 g/( kg·d) had significant inhibitory effect on pathological changes of cervical tissues. In cervicitis rat models induced by chemical substances,Zijin Huadu suppositories at the dose of1. 2 g/( kg·d) had significant inhibitory effect on vagina and cervix lesions in rats. In vitro,Zijin Huadu suppository showed obvious inhibitory effects on HSV-2,HPV16,Staphylococcus aureus,Staphylococcus albus,Staphylococcus epidermidis,group B Streptococcus,E. coli,Pseudomonas aeruginosa,Proteusbacillus vulgaris,Neisseria gonorrhoeae and Candida albicans.[Conclusions] Zijin Huadu suppository has obvious inhibitory effect in vitro on viruses and bacteria,and could obviously improve the phenol-induced cervicitis. [Objectives] To observe the pharmaceutical effect of Zijin Huadu suppository on treatment of cervicitis induced by virus, bacteria and phenol in vivo and in vitro, and provide experimental basis for evaluating its therapeutic effects for cervicitis. [Methods] Real-time RT-PCR and CPE methods were used to observe the inhibition of Zijin Huadu suppository on virus, and turbidimetry was used to observe bacteriostatic action; HE pathological section was used to observe cervicitis of rat, and enzyme-linked immunosorbent assay was used to detect virus’s expression in cervical tissue. [Results] In cervicitis rat model infected by HPV16, Zijin Huadu suppositories at the doses of 1. 2,0. 6 and0. 3 g / (kg · d) had significant inhibitory effect on HPV16 expression in cervical tissues, and the doses of 1.2 and 0. 6 g / (kg · d) had significantly inhibitory effect on pathological changes of cervical tissues. In cervicities rat models infected mixedly by Escherichia coli, Neisseria gonorrhoeae and Staphylococc us aureus, Zijin Huadu suppositories at the doses of 1.2 and 0. 6 g / (kg · d) had significant inhibitory effect on pathological changes of cervical tissues. In cervicitis rat models induced by chemical substances, Zijin Huadu suppositories at the dose In vivo, Zijin Huadu suppository manifest obvious inhibitory effects on HSV-2, HPV 16, Staphylococcus aureus, Staphylococcus albus, Staphylococcus epidermidis, group B Streptococcus, E. coli, Pseudomonas aeruginosa, Proteus bacillus vulgaris, Neisseria gonorrhoeae and Candida albicans. [Conclusions] Zijin Huadu suppository has in vitro on viruses and bacteria, and could obviously improve the phenol-induced cervicitis.