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以易感染枣疯病的阜平大枣为试材，对影响枣疯病病原荧光显微观察的主要因素进行了系统研究。结果表明：苯胺蓝（ａｎｉｌｉｎｅ ｂｌｕｅ）、 ＤＡＰＩ（４’，６－ｄｉａｍｉｄｉｎｏ－２－ｐｈｅｎｙｌｉｎｄｏｌｅ· ２ＨＣｓｄ１）和阿的平（ｑｕｉｎａｃｒｉｎｅ）３种荧光染料均可用于枣疯病病原鉴定，其中以 ＤＡＰＩ效果最佳。 ＤＡＰＩ用于定性分析的最适浓度为 ０．３μｇ·ｍＬ－１，用于定量分析的最适浓度为１．０μｇ·ｍＬ－１；对于尚未木质化或木质化程度很低的幼嫩组织切片应至少染色２０ｍｉｎ，对于高度木质化程度的组织切片应至少染色５０ｍｉｎ；带病原试材在４℃冰箱保存２０～３０ｄ，在５％戊二醛中固定４５～６０ｄ对观察结果无明显影响。以ＤＡＰＩ为染料对枣头、枣吊、叶柄、叶主脉、花梗、枝、树干皮、枣股、托叶刺、根、幼果以及接近成熟果实的组织切片进行了荧光显微观察，初步选定枝条作为最佳取材部位。
Taking Fuping jujube susceptible to jujube disease as the test material, the main factors affecting the pathogen fluorescence microscopic observation of jujube were studied systematically. The results showed that three kinds of fluorescent dyes, aniline blue, DAPI (4 ’, 6-diamidino-2-phenylindole · 2HCsd1) and quinacrine, could be used for the identification of jujube witches’ disease. good. The optimum concentration of DAPI for qualitative analysis was 0.3μg · mL-1 and the optimum concentration for quantitative analysis was 1.0μg · mL-1. For the young tissue sections which had not been lignified or had a low degree of lignification Should be stained for at least 20min, for a high degree of lignification of tissue sections should be stained at least 50min; pathogen specimens stored in a refrigerator at 4 ℃ 20 ~ 30d, fixed in 5% glutaraldehyde 45 ~ 60d no significant effect on the observation. Using DAPI as dye, the tissue sections of jujube, jujube, petiole, main vein, pedicel, branch, trunk, jujube, stipule, root and young fruit and the mature fruit were observed by fluorescence microscopy. Selected branches as the best access site.