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用PCR方法检测溶血肠毒素BL、肠毒素T和肠毒素S三种肠毒素基因(hblA、becT、entS)及毒素调控基因plcR在30株苏云金芽胞杆菌株(Bt)中的分布.结果表明,含有hblA基因、entS基因、becT基因及plcR基因片段的Bt菌株分别占66.7%、70%、70%和73.3%.其中,含有plcR基因的菌株都至少含有一种肠毒素基因,不含肠毒素基因的菌株也未检测到plcR基因,说明plcR基因与肠毒素基因有着密切的关系.用3个Bt菌株WB9、HD2和HD9(都含有hblA、becT、entS和plcR基因片段,HD2和HD9经RPLA与TECRA肠毒素检测试剂盒检测具有高滴度)对小白鼠进行口服急性毒性试验.结果表明,所有3种供试菌株的发酵上清液对小白鼠行为和健康没有明显影响,对内部器官(心、肝、肺等)也没有产生病理现象.图5表3参14
The distribution of the three enterotoxin genes (hblA, becT, entS) and the toxin regulatory gene plcR of thrombolytic toxin BL, enterotoxin T and enterotoxin S in 30 strains of B. thuringiensis was detected by PCR.The results showed that, The Bt strains containing hblA gene, entS gene, becT gene and plcR gene fragment accounted for 66.7%, 70%, 70% and 73.3%, respectively.Among them, the strains containing plcR gene contained at least one enterotoxin gene and no enterotoxin The results showed that the plcR gene was closely related to enterotoxin gene.The three Bt strains WB9, HD2 and HD9 (all containing hblA, becT, entS and plcR gene fragments, HD2 and HD9 by RPLA And TECRA enterotoxin test kit test for high titer) oral toxicity test of mice.The results showed that the fermentation supernatant of all three tested strains had no significant effect on the behavior and health of mice, the internal organs ( Heart, liver, lung, etc.) did not produce pathological phenomenon