以坚尼草（ＰａｎｉｃｕｍｍａｘｉｍｕｍＪａｃｑ．）有性二倍体ＧＲ２９７为材料将刚发芽露白的种子浸泡于０．１％的秋水仙素溶液中处理６ｈ，用自来水流水充分冲洗１ｈ后，播种于玻璃温室的水泥池中。处理株的根尖和花粉母细胞的检查结果：有４株被加倍（２ｎ＝３２），２株后死亡。加倍株的结实性和叶长与ＧＲ２９７没有明显差异。但株高变矮，生育期推迟，叶明显变宽。子房透明法的检查结果，为完全的有性生殖，可作为坚尼草有性杂交的中间亲本。 Germplasm (Panicum maximumJacq.) Sexual diploid GR297 material as soon as germinated white seeds soaked in colchicine 0.1% solution for 6h, washed with tap water for 1h, sowing in a glasshouse Cement pool. Treated plant tip and pollen mother cells of the test results: 4 were doubled (2n = 32), 2 died. Doubling the firmness and leaf length and GR297 no significant difference. But the plant height becomes shorter, the growth period is delayed, and the leaves are obviously widened. Ovary transparent test results for complete sexual reproduction, Kenny grass can be used as sexual intercourse parent.