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研究眼镜蛇毒镇痛组分的分离纯化方法,为寻找镇痛效果好,而无成瘾性的新型镇痛药物,在合成出可用于亲和吸附眼镜蛇毒心脏毒素层析介质的基础上,结合阳离子交换UND SphereTMS,凝胶过滤Sehpadex G-50,疏水层析Phenyl SepharoseTM CL-4B,从广东产舟山眼镜蛇毒中分离出镇痛多肽。此外,将凝胶过滤Sehpadex G-50应用于仿生亲和层析之前检测分离效果。得出在一步仿生亲和层析后,用疏水层析的方法分离效果更佳,仿生亲和层析适于第一步分离。
To study the separation and purification of analgesic components of cobra venom, in order to find a new analgesic drug with good analgesic effect and no addiction, based on the synthesis of cardiotoxin chromatography media for affinity adsorption of cobra venom, Cation exchange UND SphereTMS, gel filtration Sehpadex G-50, hydrophobic chromatography Phenyl Sepharose CL-4B, analgesic peptides were isolated from the Guangdong Zhoushan Cobra venom. In addition, the separation was performed before gel filtration Sehpadex G-50 was applied to biomimetic affinity chromatography. It is concluded that the separation by hydrophobic chromatography is better after one-step biomimetic affinity chromatography, and the biomimetic affinity chromatography is suitable for the first step separation.