论文部分内容阅读
目的通过研究了解南极海域海绵共附生放线菌的多样性以及活性次级代谢产物的合成潜力。方法采用4种分离培养基对南极海绵进行放线菌的分离培养,对分离到的菌株进行抗菌及微藻克生活性的筛选,对活性菌株的进行基于聚酮合酶、非核糖体肽合成酶和卤化酶的基因筛选。结果共分离得到59株海绵共附生放线菌,分布于9个属中,以链霉菌最多。21株具有较好的抗菌活性;6株菌表现出明显的赤潮微藻克生活性;有7株活性菌株至少含2种关键的功能基因,菌株OAct311和OAct408同时含有4种功能基因。结论研究结果揭示了南极海绵共附生放线菌物蕴含丰富的放线菌资源并且有较高的合成活性次级代谢物的潜力,具有深一步研究的价值。
Objective To understand the diversity of actinomycetes and the potential for the synthesis of active secondary metabolites in Antarctic sea sponges. Methods Antarctic sponges were isolated and cultured with four kinds of isolation media. The isolated strains were screened for antimicrobial activity and microalgal activity. The active strains were based on polyketide synthase, non-ribosomal peptide synthesis Gene Screening of Enzymes and Halobasic Enzymes. Results A total of 59 strains of sponges were found, which were distributed in 9 genera, with the most Streptomyces. 21 strains had good antibacterial activity; 6 strains showed obvious activity of red tide microalgal grams; 7 strains had at least 2 kinds of key functional genes. The strains OAct311 and OAct408 also contained 4 kinds of functional genes. Conclusions The results of this study reveal that the Antarctic sponge co-biofunging actinomycetes is rich in actinomycete resources and has high potential for the synthesis of active secondary metabolites, which has the value of further research.