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作者将照射尾蚴,用人工转化法获得3小时龄(3h)减弱童虫。另自接种10000条照射尾蚴的小鼠肺部收集8日龄(D8)减弱童虫。分别经血管(i.v)、气管(i.t)、腹膜(i.p)和皮内(i.d)4种途径接种于小鼠。免疫剂量为300条减弱童虫。每次试验用一组小鼠腹部皮肤(p.c)感染500条照射尾蚴,以估价每批虫的免疫原性。接种照射尾蚴或3h减弱童虫后5周及接种D8减弱童虫后4周测定抵抗力。即用尾浸没法以200条尾蚴攻击,5周后门静脉灌注,计数成虫。以下列公式计算抵抗力(R)。B=(C-T)/C×100％。公式中C,T分别为灌注试验小鼠及对照小鼠
The authors will be irradiated cercariae, artificial transformation obtained 3-hour-old (3h) weakened squirrels. In addition, 10,000 mice were inoculated with 10000 cercariae of mice and collected for 8 days (D8) to attenuate the worms. Mice were inoculated intraperitoneally (i.v.), trachea (i.t.), peritoneum (i.p.) and intradermal (i.d.) respectively. Immune dose of 300 to reduce the bug. Each group of mice was challenged with 500 irradiated cercariae per group of mice abdominal skin (p.c.) to assess the immunogenicity of each batch of worms. Resistance was measured after 5 weeks of inoculation with cercariae or 3 weeks after weaning and then 4 weeks after D8 weakened. That is to use tail cocoon to 200 cercariae attack, 5 weeks after portal vein perfusion, counting adult. Calculate the resistance (R) using the following formula. B = (C-T) / C × 100%. Formula C, T were perfusion test mice and control mice