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在日粮必需氨基酸模式相同条件下 ,研究不同日粮蛋白水平对草鱼肌肉、肝胰脏蛋白质周转代谢的影响。其中周转代谢参数包括 :蛋白质的合成速率 (FSR)、降解速率 (FDR)、生长速率 (FGR)、合成能力 (CS)、合成的翻译效率 (KRNA)和蛋白质沉积效率 (PRE)。试验结果表明 :1.日粮蛋白水平的增加促进了草鱼的生长 ,促进了肌肉、肝胰脏蛋白质的增长。饲料转化效率和肌肉蛋白质沉积效率在适宜蛋白水平时最高。2.肌肉FSR、FDR与日粮蛋白水平呈正相关关系 ;肝胰脏蛋白FSR不受日粮蛋白水平的影响 ,蛋白质FDR与日粮蛋白水平呈负相关关系。3.肌肉蛋白FGR的增加归因于蛋白质合成的增长较降解的增长更占优势 ,以及蛋白质合成的KRNA 的提高 ;肝胰脏蛋白FGR的增加归因于蛋白质降解的减少。4.肌肉、肝胰脏蛋白CS不受日粮蛋白水平的影响。5.肌肉PRE(生长占合成的比例 )在适宜蛋白质水平 (30 % )时最高 ,而且在该水平下饲料系数最低 ,即饲料转化率最高
The effects of different dietary protein levels on protein turnover metabolism in muscle, hepatopancreas of grass carp were studied under the same dietary essential amino acid pattern. The turnover metabolic parameters include: protein synthesis rate (FSR), degradation rate (FDR), growth rate (FGR), synthesis capacity (CS), synthetic translation efficiency (KRNA) and protein deposition efficiency (PRE). The results showed that: 1. The increase of dietary protein level promoted the growth of grass carp and promoted the growth of muscle, hepatopancreas and protein. Feed conversion efficiency and muscle protein deposition efficiency are highest at the appropriate protein level. There was a positive correlation between muscle FSR and FDR and dietary protein levels; FSR of hepatopancreas was not affected by dietary protein levels, and protein FDR was negatively correlated with dietary protein levels. 3. The increase of muscle protein FGR was attributed to the more dominant protein synthesis than the degradation, and the increase of protein synthesis KRNA; the increase of hepatic and pancreatic protein FGR was attributed to the decrease of protein degradation. Muscle, hepatopancreas protein CS is not affected by dietary protein levels. 5. Muscle PRE (growth as a percentage of composition) is highest at the appropriate protein level (30%) and has the lowest feed conversion at this level, ie, the highest feed conversion