Estimate of oxygen consumption and intracellular zinc concentration of human spermatozoa in relation

来源 :Asian Journal of Andrology | 被引量 : 0次 | 上传用户:shunniu
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Aim: To investigate the human sperm oxygen/energy consumption and zinc content in relation to motility. Methods: In washed spermatozoa from 67 ejaculates, the oxygen consumption was determined. Following calculation of the total oxygen consumed by the Ideal Gas Law, the energy consumption of spermatozoa was calculated. In addition, the zinc content of the sperm was determined using an atomic absorption spectrometer. The resulting data were correlated to the vitality and motility. Results: The oxygen consumption averaged 0.24μmol/106 sperm×24 h, 0.28μmol/106 live sperm×24 h and 0.85μmol/106 live & motile sperm×24 h. Further calculations revealed that sperm motility was the most energy consuming process (164.31 mJ/106 motile spermatozoa×24 h), while the oxygen consumption of the total spermatozoa was 46.06 mJ/106 spermatozoa×24 h. The correlation of the oxygen/ energy consumption and zinc content with motility showed significant negative correlations (r= -0.759; P<0.0001 and r=-0.441; P<0.0001, res Aim: To investigate the human oxygen consumption in the human sperm oxygen / energy consumption and zinc content in relation to motility. The calculation of the total oxygen consumed by the Ideal Gas Law, the energy consumption Results of The oxygen consumption averaged 0.24 μmol / 106 sperm × 24 h, 0.28 μmol / 106 sperm motility was the most energy consuming process (164.31 mJ / 106 motile spermatozoa × 24 h), while the oxygen consumption of the total correlation between the oxygen / energy consumption and zinc content with motility showed significant negative correlations (r = -0.759; P <0.0001 and r = -0 .441; P <0.0001, res
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