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本文研究了人参皂苷Rg1(Ginsenoside Rg1,Rg1)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetra-hydropyridine,MPTP)所致亚急性Parkinson病(PD)模型小鼠中脑黒质磷酸化c-Jun(p-c-Jun)与环氧合酶-2(cyclooxygenase-2,COX-2)表达的影响,以期探讨Rg1保护PD中脑黑质多巴胺(DA)能神经元可能的分子机制。实验采用MPTP制备亚急性PD小鼠模型。通过行为学观察,免疫组织化学SP法和免疫蛋白印迹法,观察PD模型小鼠行为学变化,中脑黑质酪氨酸羟化酶(TH)、COX-2和p-c-Jun表达水平的变化,并观察给予人参皂苷Rg1对上述变化的影响。结果显示,模型小鼠出现典型PD样症状。与对照组小鼠相比,黑质区TH阳性神经元数下降约65%(P<0.001),TH表达水平显著降低约75%;黑质区COX-2阳性细胞明显增多,p-c-Jun特异性表达于黑质区细胞核内,且COX-2与p-c-Jun表达水平均明显升高。经Rg1(10mg/kg)处理后,模型小鼠PD样症状减轻,与对照组比较,在MPTP第5次注射后7d,TH阳性细胞数和TH表达水平仅下降约15%和20%;与模型组比较,黑质区COX-2阳性细胞明显减少,p-c-Jun主要表达于细胞浆,部分表达于细胞核,表达水平也明显降低。以上实验结果表明,人参皂苷Rg1对MPTP诱导的亚急性Parkinson病小鼠中脑黑质细胞的神经保护作用可能是通过阻抑p-c-Jun核内表达,从而减弱COX-2表达及其介导的黒质区炎症反应。
This article studied the ginsenoside Rg1 (Ginsenoside Rg1, Rg1) against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (1-methyl-4-phenyl-1,2,3,6) Expression of c-Jun (pc-Jun) and cyclooxygenase-2 (COX-2) in midbrain tannin in mice with subacute Parkinson disease (PD) induced by tetra-hydropyridine (MPTP) The influence of Rg1 on the possible molecular mechanism of the protection of brain substantia nigra dopaminergic (DA) neurons in PD. The experiment used MPTP to prepare a subacute PD mouse model. Through behavioral observation, immunohistochemical SP method and Western blotting, the behavioral changes of PD model mice were observed, and the expression levels of tyrosine hydroxylase (TH), COX-2, and pc-Jun in substantia nigra were observed. And observe the effect of ginsenoside Rg1 on the above changes. The results showed that model mice developed typical PD-like symptoms. Compared with the control mice, the number of TH positive neurons in the substantia nigra decreased by approximately 65% (P<0.001), and the expression level of TH decreased by approximately 75%; COX-2 positive cells in the substantia nigra area increased significantly, and pc-Jun specific It is expressed in the nucleus of substantia nigra, and the expression levels of COX-2 and pc-Jun are significantly increased. After treatment with Rg1 (10 mg/kg), the PD-like symptoms of the model mice were alleviated. Compared with the control group, the number of TH positive cells and TH expression only decreased by about 15% and 20% at 7 days after the fifth injection of MPTP; Compared with the model group, the COX-2 positive cells in the substantia nigra decreased significantly, while pc-Jun was mainly expressed in the cytoplasm and partially expressed in the nucleus. The expression level was also significantly reduced. The above experimental results show that the neuroprotective effect of ginsenoside Rg1 on MPTP-induced subacute Parkinson’s disease may be through inhibiting pc-Jun nuclear expression, thereby weakening the expression of COX-2 and mediating it. Inflammatory response in the enamel area.