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目的:建立交泰丸的HPLC指纹图谱检测方法。方法:采用Agilent SB-AQ色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.05 mol·L-1磷酸二氢钾溶液(44∶56)(每100 ml中加十二烷基硫酸钠0.4 g,以磷酸调节p H值为3.5),流速1.0 ml·min-1,检测波长276 nm,柱温30℃,进样量5μl。结果:通过“中药色谱指纹图谱相似度评价系统2004A版”分析确定了12个共有峰,10批交泰丸的指纹图谱相似度均大于0.95。结论:该方法稳定可靠,为交泰丸质量标准建立提供了可靠的科学依据。
Objective: To establish HPLC fingerprinting method of Jiaotai Pills. Methods: Agilent SB-AQ column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate solution (44:56) Sodium 0.4 g, p H value adjusted to phosphoric acid 3.5), flow rate 1.0 ml · min-1, detection wavelength 276 nm, column temperature 30 ℃, injection volume 5μl. Results: The 12 common peaks were identified by the “Chinese Medicines Chromatographic Fingerprint Similarity Evaluation System 2004A Edition”, and the similarity of fingerprints of 10 batches of Jiaotai pills were all greater than 0.95. Conclusion: The method is stable and reliable, providing a reliable scientific basis for the quality standard of Jiaotai Pills.