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目的:探讨肿节风对60COγ射线照射后在小型猪放射性肺损伤中的防护作用和机制。方法:60头雄性巴马小型猪随机分为正常组、照射组和肿节风组,在麻醉条件下对动物行右胸单次15 Gy的照射,正常组不予照射。肿节风组于放疗前1周开始经口腔给予肿节风配方颗粒溶液(30 mg·kg-1),正常组和单照组给予等量的生理盐水,直至实验结束。在照射后4,8,12,24周,从3个组中分别随机取5头小型猪采集右肺组织,行羟脯氨酸测定检测肺组织中胶原含量;免疫印迹检测转化生长因子-β1(TGF-β1),基质金属蛋白酶-2(MMP-2)和MMP-9的蛋白表达;明胶酶谱法测定MMP-2和MMP-9的酶活性。结果:在照射后不同时间点,照射组中羟脯氨酸含量,TGF-β1,MMP-2和MMP-9的蛋白表达,MMP-2,MMP-9的酶活性较正常组明显升高(P<0.05);与照射组比较,肿节风组的羟脯氨酸含量,TGF-β1,MMP-2和MMP-9的蛋白表达,MMP-2,MMP-9的酶活性明显降低,差异具有统计学意义(P<0.05)。结论:肿节风可通过降低TGF-β1,MMP-2和MMP-9的蛋白表达和后者的酶活性,减缓放射性肺纤维化的发展,从而发挥放射防护作用。
Objective: To explore the protective effect and mechanism of swollenang on the radiation-induced lung injury in mini-pigs after 60COγ-ray irradiation. Methods: Sixty male Bama miniature pigs were randomly divided into normal group, irradiation group and Taijiefeng group. Under anesthesia, the animals received a single irradiation of 15 Gy on the right chest, while the normal group was not irradiated. In the treatment group, the treatment group received 30g · kg-1 of swollenang by oral administration one week before radiotherapy, and the normal group and the single group received the same amount of saline until the end of the experiment. At 4, 8, 12 and 24 weeks after irradiation, 5 miniature pigs were randomly selected from each of the 3 groups to collect the right lung tissue. Hydroxyproline was used to detect the collagen content in the lung tissue. Western blotting was used to detect the expression of transforming growth factor-β1 (TGF-β1), matrix metalloproteinase-2 (MMP-2) and MMP-9 were detected by enzyme-linked immunosorbent assay (ELISA). The enzyme activities of MMP-2 and MMP- RESULTS: The levels of hydroxyproline, the protein expressions of TGF-β1, MMP-2 and MMP-9 and the activities of MMP-2 and MMP-9 in irradiation group were significantly higher than those in normal group at different time points after irradiation P <0.05). Compared with the irradiation group, the content of hydroxyproline, the protein expressions of TGF-β1, MMP-2 and MMP-9 and the activities of MMP-2 and MMP-9 in the treatment group were significantly decreased Statistically significant (P <0.05). Conclusion: Arthritis can play a radioprotective effect by decreasing the protein expression of TGF-β1, MMP-2 and MMP-9 and the activity of the latter, and slowing the development of radiation-induced pulmonary fibrosis.