论文部分内容阅读
To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm entlength polym orphism(RFL P) ,nested PCR was employed in com bination with TD- PCR to am plify the gene and Eco RΙ and Hind were used to generate the RFL P fingerprinting.Target DNA fragm ents from 13of2 0 samples were successfully amplified and the relevant RFL P fingerprintings were obtained.It is concluded thatthe m ethod can be used to amplify the whole Cag A gene and Cag A gene has apparent diversity of RFL P profile.
To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm ent length polym orphism (RFL P), nested PCR was employed in com bination with TD-PCR to am plify the gene and Eco R I and Hind were used to generate the RFL P fingerprinting. DNA fragmnts from 13 of 20 samples were successfully amplified and the relevant RFL P fingerprintings were obtained. It is concluded that the m ethod can be used to amplify the whole Cag A gene and Cag A gene has apparent diversity of RFL P profile.