论文部分内容阅读
利用不同驯化条件(Soil、Soil+Fe(Ⅱ)、Soil+NO_3~-和Soil+Fe(Ⅱ)+NO_3~-)对华南水稻土进行中性厌氧条件下的富集培养,探究淹水期水稻土亚铁(Fe(Ⅱ))氧化和硝酸盐(NO_3~-)还原过程,及此过程中微生物群落的变化.结果表明,在Soil+Fe(Ⅱ)处理中,亚铁不能发生自然氧化.只有在Soil+Fe(Ⅱ)+NO_3~-处理中,Fe(Ⅱ)才能被氧化成Fe(Ⅲ);同时,Fe(Ⅱ)的存在减慢了NO_3~-的还原.利用高通量测序技术表征微生物群落组成随培养时间的变化,结果表明,Soil+Fe(Ⅱ)和Soil处理的微生物群落组成没有显著差异.在Soil+NO_3~-处理中,Pseudogulbenkiania、Flavobacterium和Rhodocyclus属成为优势菌群.在Soil+Fe(Ⅱ)+NO_3~-处理中,Zoogloea、Geothrix、Sunxiuqinia和Vulcanibacillus等属成为优势菌群,主要包括硝酸盐还原菌、Fe(Ⅱ)氧化菌和Fe(Ⅲ)还原菌.
Under different conditions of acclimation (Soil, Soil + Fe (Ⅱ), Soil + NO 3 - and Soil + Fe (Ⅱ) + NO 3 -), the paddy soils in southern China were enriched under neutral anaerobic conditions, (Fe (Ⅱ)) and nitrate (NO_3 ~) reduction in the paddy soils, and the changes of microbial communities in this process.The results showed that ferrous could not occur naturally in soil + Fe Oxidation of Fe (Ⅱ) to Fe (Ⅲ) only in the treatment of Soil + Fe (Ⅱ) + NO_3 ~ -, while the reduction of NO_3 ~ - slowed down by the presence of Fe The results showed that there was no significant difference in the composition of microbial community between Soil + Fe (Ⅱ) and Soil, and Pseudogulbenkiania, Flavobacterium and Rhodocyclus belonged to superiority in Soil + NO_3 ~ - treatment Among the soil + Fe (Ⅱ) + NO_3 ~ - treatments, Zoogloea, Geothrix, Sunxiuqinia and Vulcanibacillus were dominant bacteria, including nitrate reductant, Fe (Ⅱ) bacteria.