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为探讨氮肥对不同烤烟品种碳氮代谢关键酶活性的影响,采用大田试验测定了红花大金元(H)和K326(K)两个烤烟品种在施氮和不施氮条件下,不同生育期硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸脱氢酶(GDH)、蔗糖磷酸合成酶(SPS)和蔗糖合酶(SS)的活性变化。结果表明:对于同一品种,氮肥的使用在前期提高NR、GS活性,中期提高GDH活性;同时施氮提高前期SPS活性及后期SS活性。同一施氮处理情况下,K326的NR活性和SPS活性在前期高于红大,在碳代谢旺盛时期K326的GDH活性也要高于红大,在后期红大的GS酶活和SS活性高于K326。氮肥对不同品种烟株的氮代谢都起到促进作用;K326在前期利用氮素作用强于红大,后期氮素再利用作用却弱于后者,同时K326和红大在碳代谢过程中碳源向不同的碳水化合物分配的比例不同。因此在烤烟生长发育过程中,需根据不同品种氮素需求的特点,确保氮肥的供应充足。
In order to investigate the effect of nitrogen fertilizer on the key enzyme activities of carbon and nitrogen metabolism in different flue-cured tobacco varieties, field experiments were conducted to determine the effects of nitrogen fertilizer and nitrogen fertilizer on the growth of two flue-cured tobacco varieties, Dajin yuan (H) and K326 (K) (NR), glutamine synthetase (GS), glutamate dehydrogenase (GDH), sucrose phosphate synthase (SPS) and sucrose synthase (SS) The results showed that: for the same variety, the use of nitrogen fertilizer increased NR and GS activity in the early stage and GDH activity in the middle stage; while nitrogen application increased SPS activity and SS activity in the early stage. Under the same nitrogen application, the activities of NR and SPS of K326 were higher than those of the red at the early stage, while the activity of GDH at K326 was also higher than that of the red at the stage of carbon metabolism. The activities of GS and SS at the later stage were higher K326. Nitrogen fertilizer promoted the nitrogen metabolism of different varieties of tobacco plants. K326 was stronger than Nongdazhong in the early stage and was weaker than Nongliu in later stage. K326 and Hongda carbon The source distributes to different carbohydrates in different proportions. Therefore, in the process of growth and development of flue-cured tobacco, nitrogen supply needs to be ensured according to the characteristics of different varieties of nitrogen.