论文部分内容阅读
鼻咽癌是我国南方的常见肿瘤 .NGX6是新近克隆的定位于 9p2 1 2 2 ,在鼻咽癌组织中表达下调的基因 .初步的研究结果显示 ,NGX6基因转染鼻咽癌细胞HNE1能够延缓其生长速度 ,使肿瘤细胞更多地停留在G0 G1期 .为探索NGX6基因在鼻咽癌发病机制中的作用 ,建立了高表达NGX6的鼻咽癌细胞系 .利用包含 14 0 0 0个基因的cDNA微阵列分析了NGX6基因转染对HNE1细胞基因表达谱的影响 ,发现NGX6基因的转染能够上调p2 9、APC7、NEU1、RNasek6、αE catenin和TFⅡEα等基因的表达 ;同时也下调properdinP因子、G0S2、BAZ2B、ZHX1,OS4和PBX3等基因的表达 .研究结果提示 ,NGX6基因对鼻咽癌细胞的生物学行为的影响可能与它对一些细胞周期调控因子和转录调控因子的影响相关 .作为一种高通量的分析技术 ,cDNA微阵列为新基因的功能研究提供了重要的线索
Nasopharyngeal carcinoma is a common tumor in southern China.NGX6 is a newly cloned gene located in 9p2 1 2 2, which is down-regulated in nasopharyngeal carcinoma.Preliminary results show that NGX6 transfected nasopharyngeal carcinoma HNE1 can delay Its growth rate, the tumor cells to stay in the G0G1 phase.In order to explore the role of NGX6 gene in the pathogenesis of nasopharyngeal carcinoma, the establishment of high expression of NGX6 nasopharyngeal carcinoma cell line.Using 14O00 genes , We found that the NGX6 gene transfection could up-regulate the expression of genes p2 9, APC7, NEU1, RNasek6, αE catenin and TFⅡEα, as well as down-regulate properdinP , G0S2, BAZ2B, ZHX1, OS4 and PBX3.The results suggest that the effect of NGX6 gene on the biological behavior of NPC cells may be related to its effect on some cell cycle regulators and transcriptional regulators.As a result of A high-throughput analytical technique, cDNA microarrays provide important clues to the functional study of new genes