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目的了解含氯消毒剂对不同病毒的灭活效果差异,并对HIV灭活实验的方法进行探讨。方法试验按照2002版《消毒技术规范》规定的病毒灭活试验方法进行,分别对脊髓灰质炎病毒及HIV进行了悬液定量杀灭试验,评价实验结果。结果含有效氯500mg/L的次氯酸钠消毒液对脊髓灰质炎病毒作用20min,杀灭对数值>4.00,达到消毒效果;而对于HIV,含有效氯100mg/L的次氯酸钠溶液作用3min,即可达到消毒效果。结论作为亲脂性病毒的HIV对于含氯消毒剂的抗力要远远弱于脊髓灰质炎病毒。在HIV灭活试验中,以P24抗原为指标来确定病毒的感染滴度要比用观察细胞的病变方法来判断病毒灭活的效果更为确定、客观和科学。
Objective To understand the differences of inactivation effects of different chlorine disinfectants on different viruses and explore the methods of HIV inactivation experiments. Methods The test was conducted according to the virus inactivation test method stipulated in the “Technical Specifications for Disinfection” of the edition of “2002”. Quantitative suspension test of poliovirus and HIV was conducted to evaluate the experimental results. Results With sodium hypochlorite disinfectant containing 500mg / L of effective chlorine for 20min, kill logarithm> 4.00, to achieve disinfection; and for HIV, containing chlorine 100mg / L of sodium hypochlorite solution for 3min, you can achieve disinfection effect. Conclusions HIV as a lipophilic virus is far less resistant to chlorinated disinfectants than poliovirus. In the HIV inactivation test, P24 antigen as an indicator to determine the virus titer than the observation of cell lesions to determine the effect of virus inactivation more certain, objective and scientific.