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乙型肝炎病毒(HBV)慢性感染与发主肝细胞癌(HCC)的关系最初是从下述两方面的流行病学调查结果中得到支持的:1.乙型肝炎表面抗原(NBsAg)慢性携带状态和肝细胞癌发生率的地理分布相似;2.HCC患者皿清中HBV标记物的发现率高于普通人群。此外,在HCC患者某些肝肿瘤细胞中发现HBsAg,也支持HBV与HCC的相互关系。尽管对HBV感染的血清学特异性反应已得到很好的阐明,但几乎还未了解有关本病不同阶段中的病毒周期及其与HCC夫系的性质.这主要有两万面的原因:1.HBV不能成功地在细胞培养中传代.因而大大阻碍了对此病毒进行分于生物学的研究;2.HBV的实验感染仅在少数灵长类(如黑猩猩)动物中才能获得成功,但黑猩猩发病的病程不同于人体。黑猩猩主要发生慢性持续性肝炎(CPH),迄今尚未发现HCC。在土拨鼠、松鼠和鸭体内研究与HBV有关的病毒,成为适于研究宿主与病毒相互关系的实验室模型。但是在这些动物中观察到的肝病变也稍与人的疾病有些不同。例如,未见到松鼠发生HCC以及土拨鼠的HCC与急性肝炎或慢性活动性肝炎和病毒的大量增殖有关,但与在人体观察到的肝硬化无夫. 根据Southern Blot氏方法,研究HBV与HCC关系的一种方法是,应用克隆的乙型肝炎病毒DNA作为一种特异性探子,来探查乙型肝炎病毒DNA的序列。该技术可测定肝细胞内病毒DNA的状态,在宿主细胞内的DNA为游离状态或结合状态。这些结果为研究HBV对原发性肝癌的作用以及研究病毒的周期提
The relationship between chronic hepatitis B virus infection and primary hepatocellular carcinoma (HCC) was initially supported by epidemiological findings from two aspects: 1. Chronic hepatitis B surface antigen (NBsAg) The distribution of HBV markers in HCC patients was higher than that in the general population. In addition, HBsAg is found in some liver tumor cells of HCC patients and also supports the relationship between HBV and HCC. Although the serological specific response to HBV infection has been well elucidated, there is little known about the virus cycle in different stages of the disease and the nature of the relationship with the HCC .There are two main reasons for this: 1 .HBV can not be successfully passaged in cell culture, which greatly hindered the biology of the virus; 2. Experimental infection with HBV is successful only in a few primates (eg, chimpanzees), but chimpanzees The course of the disease is different from the human body. Chronic persistent hepatitis (CPH) predominates in chimpanzees, and so far no HCC has been found. The study of HBV-related viruses in woodchucks, squirrels and ducks has become a laboratory model suitable for studying host-virus interactions. However, the liver lesions observed in these animals are also slightly different from those in humans. For example, no HCC occurrence in squirrels and groundhog HCC were seen to be associated with massive proliferation of acute hepatitis or chronic active hepatitis and viruses, but not with cirrhosis observed in humans.According to Southern Blot’s method, HBV and One way to understand the HCC relationship is to use the cloned hepatitis B virus DNA as a specific probe to probe the sequence of the hepatitis B virus DNA. This technique measures the state of viral DNA in hepatocytes, with the DNA in the host cell either in a free or bound state. These results are to investigate the effect of HBV on primary liver cancer and to investigate the periodicity of the virus