过去十年来,分子生物学的发展主要归功于那些必需技术的发展,而这些技术的中心则是体外放射标记高比活的DNA。目前,使用两种方法制备高比活的DNA分子——通过切口前移(nick-translation)标记双链DNA和通过引用单链DNA模板进行的DNA合成。现在,一种新的以特异重组的质 Over the past decade, the development of molecular biology has largely been attributed to the development of the necessary technologies, which center on in vitro radio-labeled DNA with high specific activity. Currently, two methods are used to prepare high specific activity DNA molecules - labeling of double stranded DNA by nick-translation and DNA synthesis by referencing single stranded DNA templates. Now, a new kind of substance with specific recombination