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通过生物技术手段,把无性繁殖应用到生产上的困难之一是受再生和增殖能力的限制。我们在水稻“金刚30”体细胞培养中选择到一块再生和增殖能力极强的材料,分出3～5个这种芽头到新鲜培养基上培养20天左右,就可增殖到百十个以上。经三年多的继代培养仍保持着这种特性,是一个很有价值的材料。但用常规方法始终不能使他生根,影响到此材料的进一步研究和利用,为此经过一系列试验,弄清了其生根特异性。 1.渗透压的影响:在固体培养基上,不
One of the difficulties in applying asexual reproduction to production through biotechnological means is that it is limited by the ability to regenerate and proliferate. We selected a piece of rice in the “King Kong 30” somatic cell culture to a very strong ability of regeneration and proliferation of materials, divided into 3 to 5 of this bud head to fresh medium for about 20 days, you can proliferate to a hundred the above. After more than three years of subculture still maintain this feature, is a very valuable material. However, using conventional methods can not always make him take root, affecting the further research and utilization of this material, for which a series of experiments to clarify the rooting specificity. 1. Osmotic pressure effects: on solid medium, no