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目的:研究龙牡壮骨颗粒对小鼠成骨细胞株MC3T3-E1增殖、分化和矿化的影响。方法:以小鼠成骨细胞株MC3T3-E1作为药物筛选的体外模型;采用终浓度分别为1,5,10,25,50μg/ml的龙牡壮骨颗粒溶液干预,MTT法检测细胞增殖率;同时检测药物作用不同时间后的碱性磷酸酶(ALP)活性;茜素红染色和Von Kossa钙化染色检测不同浓度药物对细胞钙化的影响。结果:龙牡壮骨颗粒溶液在5~50μg/ml范围内培养24和48 h可显著促进小鼠成骨细胞增殖;龙牡壮骨颗粒溶液在5~50μg/ml范围内培养48和72 h显著提高MC3T3-E1细胞ALP活性。龙牡壮骨颗粒浓度为10μg/ml和50μg/ml时,茜素红染色钙化结节显著增多,药物浓度在10~50μg/ml范围内,Von Kossa染色钙化结节显著增多。结论:龙牡壮骨颗粒可提高MC3T3-E1增殖、分化及矿化的能力。
Objective: To investigate the effect of Longmuzhuanggu granule on the proliferation, differentiation and mineralization of mouse osteoblastic cell line MC3T3-E1. Methods: The murine osteoblast cell line MC3T3-E1 was selected as an in vitro model for drug screening. The cells were treated with Longmu Zhuanggu Granules at the concentrations of 1, 5, 10, 25 and 50μg / ml respectively. The cell proliferation rate Alkaline phosphatase (ALP) activity was measured at different time points after drug treatment. Alizarin red staining and Von Kossa calcification were used to detect the effects of different concentrations of drugs on cell calcification. Results: Longmu Zhuanggu granule solution cultured in the range of 5 ~ 50μg / ml for 24 and 48 hours could significantly promote the proliferation of mouse osteoblasts. Longmu Zhuanggu granule solution was cultured in the range of 5 ~ 50μg / ml for 48 and 72 hours Significantly increase the ALP activity of MC3T3-E1 cells. Calculi nodules significantly increased with the concentration of Longmu Zhuanggu particles at 10μg / ml and 50μg / ml, and the number of calcified nodules increased significantly with Von Kossa staining in the range of 10-50μg / ml. Conclusion: Longmu Zhuanggu granules can enhance the proliferation, differentiation and mineralization ability of MC3T3-E1.