论文部分内容阅读
目的探讨还原型辅酶Ⅱ(NADPH)氧化酶在大鼠心肌梗死后左室重构中的作用。方法 25只SD大鼠随机分为四组:心肌梗死组(A组,7只)、心肌梗死+夹竹桃麻素15mg.kg-1.d-1组(B组,6只)、假手术组(C组,6只)和假手术+生理盐水组(D组,6只)。6周后处死大鼠,称取心脏和左室重量,ELISA法检测胶原蛋白Ⅰ、Ⅲ含量,TUNEL法检测心肌细胞凋亡并分析其与左室射血分数(LVEF)的关系。结果与A组相比,B、C、D组心脏及左室重量指数、胶原蛋白Ⅰ含量及Ⅰ/Ⅲ比值、心肌细胞凋亡指数均明显降低(P<0.05或P<0.01),而LVEF则显著增高(P<0.01)。心肌细胞凋亡指数与LVEF呈负相关(r=-0.757,P<0.01)。结论心肌梗死后NADPH氧化酶激活可能是导致左室重构的重要原因之一。
Objective To investigate the role of reduced coenzyme Ⅱ (NADPH) oxidase in left ventricular remodeling after myocardial infarction in rats. Methods Twenty-five Sprague-Dawley rats were randomly divided into four groups: myocardial infarction (group A, n = 7), myocardial infarction + apocynin 15 mg.kg-1.d-1 The operation group (C group, 6 rats) and sham operation + saline group (D group, 6 rats). After 6 weeks, the rats were sacrificed and the heart and left ventricular weight were weighed. The contents of collagen Ⅰ and Ⅲ were detected by ELISA. The apoptosis of cardiomyocytes was detected by TUNEL and the relationship between them and left ventricular ejection fraction (LVEF) was analyzed. Results Compared with group A, the cardiac and left ventricular mass index, collagen Ⅰ content and Ⅰ / Ⅲ ratio, myocardial apoptosis index were significantly decreased in groups B, C and D (P <0.05 or P <0.01), while LVEF Was significantly higher (P <0.01). Myocardial apoptosis index was negatively correlated with LVEF (r = -0.757, P <0.01). Conclusion The activation of NADPH oxidase after myocardial infarction may be one of the important causes of left ventricular remodeling.