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目的研究阿伦膦酸钠(AL)对在体和离体大鼠动脉钙化的影响。方法4周龄SD雄性大鼠18只随机分为AL组、钙化组和正常组,前2组分别给予皮下注射华法林15mg.100g-1.12h-1,4d;维生素D3300000U.kg-1.24h-1,3d,以制备大鼠动脉钙化。AL组在钙化模型制备之前4d给予AL1mg.kg-1.24h-1皮下注射。细胞分为AL10-9、10-7和10-5mol/L组,钙化组和正常组。结果AL组大鼠主动脉vonKossa染色黑色深染结构减少。AL治疗的各组茜素红S染色发现钙结节计数较钙化组减少[(6.8±2.7,6.2±4.2,5.3±2.4)%比(7.4±3.8)%],细胞钙沉积含量减少[(5.2±1.2,4.8±1.7,3.5±1.8)%比(5.6±1.6)%],ALP活力和细胞增殖均降低,并呈剂量依赖性。结论AL能抑制大鼠动脉钙化。
Objective To investigate the effect of alendronate (AL) on arterial calcification in vivo and in vitro. Methods Four male Sprague-Dawley rats of 4 weeks old were randomly divided into AL group, calcification group and normal group. The first two groups were subcutaneously injected with warfarin 15mg.100g-1.12h-1,4d, vitamin D3300000U.kg-1.24h -1,3 d to prepare rat arterial calcifications. The AL group was given subcutaneous injection of AL1mg.kg-1.24h-1 4d before the calcification model was prepared. Cells were divided into AL10-9,10-7 and 10-5mol / L group, calcification group and normal group. Results The dark staining of von Kossa staining in aorta of AL rats decreased. Alizarin red S staining showed that the calcium count in the AL group was significantly lower than that in the calcification group [(6.8 ± 2.7,6.2 ± 4.2,5.3 ± 2.4)% vs (7.4 ± 3.8)%] 5.2 ± 1.2, 4.8 ± 1.7 and 3.5 ± 1.8%, respectively. The ALP activity and cell proliferation decreased in a dose-dependent manner. Conclusion AL can inhibit arterial calcification in rats.