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目的:观察大黄-桃仁对术后粘连性肠梗阻模型大鼠炎性细胞因子水平及肠黏膜屏障的影响，探讨其作用机制。方法:将60只SD大鼠按随机数字表法分为正常组，假手术组，模型组，大黄-桃仁低、中、高剂量组，每组10只。除正常组和假手术组外，其余各组大鼠制备术后粘连性肠梗阻模型。造模后第1天开始，大黄-桃仁低、中、高剂量组灌胃0.2、0.6、1.8 g/ml的大黄-桃仁水煎剂，正常组、假手术组、模型组灌胃等体积的生理盐水，1次/d，连续灌胃7 d。观察粘连评分，采用ELISA法检测大鼠血清IL-1β、D-乳酸(D-lactic acid, D-LA)、二胺氧化酶(diamine oxidase, DAO)和血管内毒素(endotoxin, ET)含量；免疫组织化学染色法观察大鼠肠黏膜分泌型免疫球蛋白A(secretory immunoglobulin A, SIgA)、CD4n +T和CD8n +T表达。n 结果:与模型组比较，大黄-桃仁低、中、高剂量组粘连评分降低(n P<0.05)，血清IL-1β[(8.66±1.07)ng/L、(8.15±1.23)ng/L、(7.99±1.11)ng/L比(14.08±2.54)ng/L]水平降低(n P<0.01)，肠黏膜SIgA[(1.38±0.15)、(2.87±1.17)、(2.79±0.80)比(0.65±0.12)]表达升高(n P<0.01)；大黄-桃仁中、高剂量组血清D-LA[(8.57±1.73)mg/L、(7.13±1.75)mg/L比(14.58±2.81)mg/L]、ET[(77.39±6.83)mg/ml、(50.49±7.80)mg/ml比(138.22±7.79)mg/ml]水平降低(n P<0.01)，肠黏膜CD4n +T[(2.61±0.83)、(2.91±1.62)比(1.15±0.98)]和CD8n +T[(2.88±0.69)、(3.01±1.86)比(1.26±0.74)]表达升高(n P<0.01)。n 结论:大黄-桃仁水煎剂可改善大鼠肠黏膜通透性，有效保护肠黏膜免疫屏障，从而减轻炎性反应，防治粘连性肠梗阻。“,”Objective:To observe the effect of n Rhei Radix et Rhizoma and n Persicae Semen on inflammatory cytokines and intestinal mucosal barrier in rats with postoperative adhesive intestinal obstruction, and to explore its mechanism.n Methods:Sixty SD rats were randomly divided into normal group, sham operation group, model group, low, medium and high dose of n Rhei Radix et Rhizoma and n Persicae Semen group. Except for the normal group and the sham operation group, and the other animals groups were established the model of postoperative adhesive intestinal obstruction. n Rhei Radix et Rhizoma and n Persicae Semen low, medium and high dose groups were perfused with n Rhei Radix et Rhizoma and n Persicae Semen decoction with the concentration of 0.2, 0.6 and 1.8 g/ml, the normal group, sham operation group and model group were gavaged with equal volume of sterile saline from the first day after the operation, once a day. After corresponding treatment, the adhesion score was observed on the 7th day after the operation, the contents of interleukin-1 β (IL-1β), D-lactic acid (D-LA), diamine oxidase (DAO) and vascular endotoxin (ET) in serum were detected by ELISA method, and the expression of SIgA, CD4 n +T and CD8n +T cells in intestinal mucosa were assessed by immunohistochemical method.n Results:Compared to the model group, the adhesion score in the low, medium and high dose of n Rhei Radix et Rhizoma and n Persicae Semen group significantly decreased (n P<0.05), the level of serum IL-1β [(8.66 ± 1.07) ng/L, (8.15 ± 1.23) ng/L, (7.99 ± 1.11) ng/Ln vs. (14.08 ± 2.54) ng/L] significantly decreased (n P<0.01), the expression of SIgA (1.38 ± 0.15, 2.87 ± 1.17, 2.79 ± 0.80n vs. 0.65 ± 0.12) in intestinal mucosa in the low, medium and high dose of n Rhei Radix et Rhizoma and n Persicae Semen group significantly increased (n P<0.01). The levels of serum D-LA [ (8.57 ± 1.73) mg/L, (7.13 ± 1.75) mg/Ln vs. (14.58 ± 2.81) mg/L], ET [ (77.39 ± 6.83) mg/ml, (50.49 ± 7.80) mg/mln vs. (138.22 ± 7.79) mg/ml] significantly decreased (n P<0.01), the expression of CD4n +T (2.61 ± 0.83, 2.91 ± 1.62n vs. 1.15 ± 0.98) and CD8n +T (2.88 ± 0.69, 3.01 ± 1.86n vs. 1.26 ± 0.74) cells in intestinal mucosa in the medium and high dose of n Rhei Radix et Rhizoma and n Persicae Semen group significantly increased (n P<0.01).n Conclusion:Rhei Radix et Rhizoma and n Persicae Semen decoction can improve intestinal mucosal permeability, protect intestinal mucosal immune barrier and reduce inflammatory reaction in the treatment of adhesive intestinal obstruction.n