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目的 利用多种方法检测金属蛋白酶MMP - 9基因与人黑色素瘤细胞生长的相关性。方法 利用细胞记数、MTT、3H -thymidine掺入实验等方法检测分别转染正义和反义MMP - 9CDNA后人黑色素瘤细胞生长速度的改变。结果 转染反义基因后WM 4 5 1细胞生长速度受到明显抑制 (P <0 .0 5 ) ;转染正义基因后WM 35细胞生长速度受到明显促进 (P <0 .0 5 )。而运用四环素可以抑制四环素负调控逆转录病毒载体上的外源基因的表达 ,其结果与对照细胞相似。结论 反义MMP - 9基因使人黑色素瘤细胞的生长能力受到一定程度的抑制 ,正义MMP - 9基因使人黑色素细胞的生长能力受到一定程度的促进。说明MMP - 9在人黑色素瘤细胞生长过程中起重要作用。同时 ,四环素负调控逆转录病毒载体可以调控外源基因的表达
Objective To detect the correlation between MMP - 9 gene and the growth of human melanoma cells by various methods. Methods The growth rate of human melanoma cells was detected by cell counting, MTT and 3H-thymidine incorporation assay. Results The growth of WM 4 51 cells was significantly inhibited after antisense gene transfection (P <0.05). The growth of WM 35 cells was significantly enhanced after transfection of sense gene (P <0.05). The use of tetracycline can inhibit the expression of foreign genes on the tetracycline negative regulatory retroviral vector, the results are similar to the control cells. Conclusion The antisense MMP - 9 gene inhibits the growth of human melanoma cells to a certain extent. The growth of human melanocytes is enhanced to some extent by the MMP - 9 gene. It shows that MMP - 9 plays an important role in the growth of human melanoma cells. Meanwhile, tetracycline negative regulatory retroviral vector can regulate the expression of foreign genes