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近二年,印度的Murfy和Adiga等人的工作表明,在妊娠大鼠血清中存在着一种妊娠特异性、高亲和性的核黄素运载蛋白。Adiga等人的实验显示,该运载蛋白对维持妊娠和胚胎健康发育有极大重要性,并报道了对大鼠的RCP进行免疫中和可立即导致妊娠终止。作者用亲和性层析法首次分离到RCP,该蛋白分子量为185,000,有两个不等的亚单位,分子量分别为86,000和96,000。目前已在鸟类、鸡、猴子、牛及人等的血清中发现有相似的运载蛋白。作者用妊娠大鼠作为模型,把注入~(14)C-核黄素的妊娠大鼠用抗鸡RCP抗血清进行免疫中和,结果发现,在妊娠第6天和16天之间注射抗血清的动物中,24小时至48小时内有大量阴道出血,接着就是100%的流产。在妊娠11天时注射大鼠RCP抗血清也可得到相似结果。同时在处理组中发现3—6小时后~(14)C-核黄素的摄入量有明
In the past two years, the work of Murfy and Adiga in India showed that there is a pregnancy-specific and high-affinity riboflavin-carrying protein in the serum of pregnant rats. Experiments by Adiga et al. Show that the carrier protein is of great importance for the maintenance of healthy pregnancy and embryonic development and reports that immunosuppression of RCP in rats can immediately lead to the termination of pregnancy. The authors first isolated RCP using affinity chromatography, which has a molecular weight of 185,000 and two unequal subunits with molecular weights of 86,000 and 96,000, respectively. At present, similar carrier proteins have been found in the sera of birds, chickens, monkeys, cattle and humans. Using pregnant rats as a model, the pregnant rats infused with ~ (14) C-riboflavin were immunized with anti-chicken RCP antiserum and found that antisera were injected between days 6 and 16 of gestation Of animals, there is a lot of vaginal bleeding within 24 hours to 48 hours, followed by 100% abortion. Similar results were obtained with rat RCP antisera injected at 11 days gestation. At the same time, the intake of ~ (14) C-riboflavin was found in the treatment group after 3-6 hours