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目的 :研究代谢型谷氨酸受体 (mGluRs)激动剂引起大鼠向对侧旋转时介导的受体亚型。方法 :大鼠纹状体内微量注射mGluRs激动剂或拮抗剂 ,观察大鼠的意识、行为变化 ,并于给药后 6h测定旋转活动。结果 :mGluRs非亚型特异的激动剂tACPD (5 0 0、10 0 0nmol)纹状体内注射引起大鼠向对侧旋转 ,mGluRs的非竞争性拮抗剂L -AP3 、竞争性拮抗剂MCPG及抑制细胞内钙释放的胆罗啉均可减轻tACPD引起的旋转。I组mGluRs的特异性激动剂DHPG (5 0 0nmol)纹状体内注射也引起大鼠向对侧旋转 ,MCPG及mGluR1的拮抗剂LY36 7385及mGluR5的拮抗剂MPEP均可拮抗DHPG引起的旋转。预先腹腔注射利血平 (5mg/kg)可阻断DHPG的作用。结论 :I组mGluRs激动引起大鼠向对侧旋转 ,此作用可能与细胞内钙释放有关及依赖于多巴胺的存在。
OBJECTIVE: To study the receptor subtypes mediated by rat gyroglidalgic response to metabotropic glutamate receptor (mGluRs) agonists. Methods: The mGluRs agonists or antagonists were injected into the striatum of rats to observe the changes of consciousness and behavior in rats. The rotational activity was measured 6h after administration. RESULTS: Striatal injections of tACPD (500 microg / 100 nmol), a non-subtype-specific agonist of mGluRs, caused contralateral rotation, L-AP3, a competitive antagonist of mGluRs, and MCPG, a competitive antagonist The intracellular calcium release of choledin can reduce the rotation caused by tACPD. Injection of DHPG (500 nmol), a specific agonist of group I mGluRs, also caused contralateral rotation in rats. Both LY36 7385, an antagonist of mGluR1 and MPEP, an antagonist of mGluR5, antagonized the DHPG-induced rotation. Preperitoneal injection of reserpine (5mg / kg) can block the role of DHPG. CONCLUSIONS: Group I mGluRs agonist caused contralateral rotation in rats, which may be related to intracellular calcium release and the presence of dopamine.