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目的观察c-Myc反义寡核苷酸(c-Myc ASODN)对人肝癌Bel-7402细胞增殖的双重调节作用,探讨c-Myc基因的生物学功能。方法台盼兰拒染细胞计数,CCK-8细胞检测试剂盒检测各组细胞增殖抑制率,计算单纯黄连素组及合用c-Myc ASODN后IC50。结果单纯黄连素及c-Myc ASODN均能明显抑制细胞的增殖(P<0.05),将黄连素合用c-Myc ASODN后,细胞增殖抑制率低于单用黄连素组,但仍高于c-Myc ASODN组。黄连素作用Bel-7402细胞的IC50值在合用c-Myc ASODN后增加1.42倍。结论c-Myc基因在Bel-7402细胞细胞生长环境良好时可促进细胞的生长,在细胞生长环境不利的情况下则诱导细胞的的凋亡,抑制细胞的生长。依据环境不同,对Bel-7402细胞增殖具有不同的调节作用,c-Myc ASODN通过下调c-Myc基因的表达,在不同的环境下亦表现出不同的生物学效应。c-Myc ASODN与黄连素合用能明显降低Bel-7402细胞对黄连素的敏感性。
Objective To investigate the dual regulatory effect of c-Myc ASODN on the proliferation of human hepatocellular carcinoma Bel-7402 cells and to explore the biological function of c-Myc gene. Methods The cell counts of trypan blue exclusion were detected. The cell proliferation inhibition rate of each group was detected by CCK-8 cell test kit. IC50 was calculated in pure berberine group and combined with c-Myc ASODN. Results Both berberine and c-Myc ASODN could significantly inhibit the proliferation of cells (P <0.05). The inhibitory rate of berberine combined with c-Myc ASODN was lower than that of berberine alone, but still higher than that of c- Myc ASODN group. The IC50 value of berberine-treated Bel-7402 cells increased by 1.42-fold after co-administration of c-Myc ASODN. Conclusion c-Myc gene can promote cell growth when Bel-7402 cell growth is good, and induce cell apoptosis and inhibit cell growth under unfavorable cell growth environment. Depending on the environment, c-Myc ASODN has different regulatory effects on Bel-7402 cell proliferation. C-Myc ASODN shows different biological effects in different environments by down-regulating c-Myc gene expression. c-Myc ASODN and berberine can significantly reduce the Bel-7402 cell sensitivity to berberine.