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目的建立皂角刺的HPLC指纹图谱,并同时测定皂角刺中7,3′,5′-三羟基-5-甲氧基二氢黄酮醇、3,3′,5,5′,7-五羟基二氢黄酮醇、槲皮素3种黄酮类成分的量。方法采用Waters symmetry C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈(A)-0.1%甲酸水(B)溶液,梯度洗脱,0~5 min,5%~13%A;5~20 min,13%~16%A;20~45 min,16%~20%A;45~50 min,20%~25%A;50~65 min,25%A;65~80 min,25%~40%A;体积流量0.9 m L/min;柱温25℃;检测波长338 nm。结果对13批皂角刺药材进行研究,所得HPLC指纹图谱共标定14个共有峰,并对其中3个成分进行了测定,其质量浓度分别在0.091 3~5.840 0、0.176 3~11.280 0、0.014 0~0.895 0 mg/m L与峰面积呈良好线性关系(r≥0.999 2),平均加样回收率为98.97%~99.66%,RSD<2.5%。结论该法具有较好的稳定性和重复性,为皂角刺药材的质量控制提供科学依据。
OBJECTIVE To establish the HPLC fingerprints of Gleditsia sinensis, and to determine the content of 7,3 ’, 5’-trihydroxy-5-methoxy-dihydroflavonol, 3,3’, 5,5 ’, 7- Five hydroxyl dihydroflavonols, quercetin three kinds of flavonoid content. Methods Waters symmetry C18 (250 mm × 4.6 mm, 5 μm) column was used. The mobile phase consisted of acetonitrile (A) - 0.1% formic acid in water (B) 5-20 min 13% -16% A 20-45 min 16-20% A 45- 50 min 20-25% A 50-65 min 25% A 65- 80 min, 25% ~ 40% A; volume flow 0.9 m L / min; column temperature 25 ℃; detection wavelength 338 nm. Results Thirteen batches of Gleditsia sinensis were studied. The HPLC fingerprint was used to determine 14 common peaks and three of them were determined. Their mass concentrations were 0.091 3 ~ 5.840 0, 0.176 3 ~ 11.280 0, 0.014 The calibration curve showed a good linearity (r≥0.999 2) with the average recovery of 98.97% -99.66% and RSD <2.5% with a range of 0 ~ 0.895 0 mg / m L. Conclusion The method has good stability and repeatability, and provides a scientific basis for the quality control of Radix Glabra.