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[目的]猫尾草早熟、晚熟类型在成熟期上存在明显的差异,该研究可为猫尾草种质资源在高寒牧区的利用提供依据。[方法]过氧化物酶同工酶和电导率法配合Logistic方程确定早熟、晚熟猫尾草冰冻半致死温度。[结果]4份材料的过氧化物酶同工酶谱带数为3~11条,相对迁移率分布在0.169 0~0.704 2,早熟、晚熟类猫尾草之间的酶谱相似系数较小。早熟类2份材料的酶带有差异,酶谱相似系数为0.750,晚熟类2份材料的酶带也有差异,酶谱相似系数为0.706。早熟、晚熟猫尾草LT50分别为-34.29、-33.97℃,绝对数值仅相差0.32℃。[结论]早熟类与晚熟类猫尾草的遗传相似性较小,确证了早熟、晚熟类的差异性大,早熟、晚熟猫尾草的抗寒性没有显著差异,它们都能在引种地安全越冬。
[Objective] The research showed that there are obvious differences in the early maturity and late maturity types of Timothy in the mature period. This research can provide the basis for the utilization of Timothy germplasm in the alpine pastoral area. [Method] Peroxidase isoenzyme and conductivity method were combined with Logistic equation to determine the semi - lethal temperature of premature and late mature Timothy. [Result] The peroxidase isozyme band numbers of 4 materials ranged from 3 to 11, and the relative mobility was between 0.169 0 and 0.704 2. The similarity coefficients of the enzymes of early-ripening and late-ripening Timothy were lower . There were differences in the enzyme bands between the two precocious species, with the similarity coefficient of 0.750, the enzyme bands of two late-maturing varieties also had differences, and the similar coefficient of zymogram was 0.706. Early maturing, late-maturing Timothy LT50 were -34.29, -33.97 ℃, the absolute value of only a difference of 0.32 ℃. [Conclusion] The genetic similarity between the precocious species and the late-ripened species Timothy was smaller, which confirmed the difference between the precocious and the late-ripened species. There was no significant difference in the cold resistance between the precocious and the late-ripened Timothy. All of them could be safely introduced Winter.