论文部分内容阅读
目的:探讨CTLA-4基因多态性位点-1722T/C和CT60G/A与中国北方汉族妇女乳腺癌易感性的关系。方法:采用聚合酶链反应(PCR)和限制性片段长度多态性方法,对328例中国北方汉族乳腺癌患者和327例正常对照者进行CT-LA-4基因-1722位点和CT60位点多态性检测。结果:乳腺癌患者CTLA-4基因CT60位点G等位基因频率在乳腺癌患者组中明显高于正常对照组(28.7%比23.5%;P=0.0352,OR=1.30,95%CI=1.02~1.67);-1722C-CT60A单体型在对照组中的频率大于病例组中的频率,有明显差异(P=0.0283,OR=0.77,95%CI=0.97~0.61),而在-1722位点基因型频率、等位基因频率与对照组比较差异无统计学意义(P>0.05)。结论:CTLA-4基因多态性-1722和CT60两个位点与我国北方汉族妇女乳腺癌发病存在一定的相关性。
Objective: To investigate the relationship between polymorphisms of CTLA-4 gene -1722T / C and CT60G / A and susceptibility to breast cancer in Han women from northern China. Methods: The CT-LA-4 gene -1722 locus and CT60 locus were detected in 328 Chinese Han patients with breast cancer and 327 normal controls by polymerase chain reaction (PCR) and restriction fragment length polymorphism Polymorphism detection. Results: The frequency of allele G at CT60 locus in breast cancer patients was significantly higher than that in controls (28.7% vs 23.5%; P = 0.0352, OR = 1.30, 95% CI = 1.02 ~ 1.67). The frequency of 1722C-CT60A haplotype in the control group was significantly higher than that in the case group (P = 0.0283, OR = 0.77, 95% CI = 0.97-0.61) There was no significant difference in genotype frequency and allele frequency between the two groups (P> 0.05). CONCLUSION: The polymorphisms of CTLA-4 gene -1722 and CT60 are associated with the incidence of breast cancer in Han women in northern China.