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目的:探讨纤维蛋白单体引起细胞伸展的机理.方法:通过2MOL/LNABR溶解纤维蛋白制成纤维蛋白单体.采用细胞粘附及抑制方法观察细胞伸展状况.结果:人神经胶质瘤细胞不能在固定的纤维蛋白原上伸展,但能在纤维蛋白单体上伸展,此伸展过程不能被外来的纤维蛋白原、片段E和D抑制,但能被纤维蛋白单体和RGD(精氨酸－甘氨酸－天冬氨酸)序列所抑制.结论:纤维蛋白单体引起细胞伸展的可能机理为纤维蛋白单体α链上的两个RGD序列Aα(95－97)和Aα(572－574)暴露,从而促使纤维蛋白单体与细胞作用,调节细胞伸展“,”Objective: This paper was designed to analyse the molecular mechanism of cell spreading induced by fibrin monomer. Methods: We prepared fibrin monomer dissolving fibrin in 2 mol/L sodium bromide. The cell spreading was observed by a new cell adhesion assay system. Results: Human neuroglioma cells could not spread on fixed fibrinogen but extended on fibrin monomer. The extension was not inhibited by exogenous fibrinogen, fragment E and D; but was blocked by fibrin monomer and RGD synthetic peptide. Conclusi...