论文部分内容阅读
使用不同剂量(2、4、6 mg/kg)的N-亚硝基-N-氨基甲酸乙酯(NMU)诱发大鼠急性肺损伤。分析了在急性肺损伤情况下大鼠肺成份、即肺泡游离细胞成份、细胞外肺表面活性物质成份,层状体和微粒体成份的磷脂特性及其变化。以6mg/kg给大鼠皮下注射NMU后第2天,肺泡空间成份的磷脂含量明显减少,细胞内成份没有变化,而在4mg/kg的剂量下未见到类似的结果,甚至在2mg/kg时磷脂的含量稍有增加。肺各成份中磷脂酰胆碱没有受到NMU的影响,但磷脂酰肌醇和鞘磷脂增多,磷脂酰甘油减少,即PG/PI的比率明显降低,PC/SM的比率也有所下降。
Acute lung injury in rats was induced using different doses (2,4,6 mg / kg) of N-nitroso-N-urethane (NMU). The characteristics of phospholipids and their changes of lung components in rat lung, such as alveolar free cell components, extracellular lung surfactant substances, lamellar and microsomal components, were analyzed in acute lung injury. On days 2 after subcutaneous injection of NMU into rats at 6 mg / kg, alveolar space components had a significantly reduced phospholipid content and no intracellular components, whereas similar results were not seen at a dose of 4 mg / kg even at 2 mg / kg Phospholipid content slightly increased. Phosphatidylcholine in the lungs was not affected by NMU, but increased phosphatidylinositol and sphingomyelin, decreased phosphatidylglycerol (PG / PI), and decreased PC / SM ratio.