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目的观察通络醒脑泡腾片对快速老化小鼠亚系8(SAMP8)海马的Neu N蛋白及其超微结构的影响。方法将SAMP8小鼠随机分为SAMP8组、安理申组、通络醒脑泡腾片组(高、中、低剂量),另取抗快速老化小鼠亚系1(SAMR1)作为对照组,分组后连续给药60 d,采用Morris水迷宫和避暗法评价其学习记忆的改变;采用免疫组化法考察海马Aβ和Neu N蛋白的表达;采用电镜法考察海马的超微结构。结果通络醒脑泡腾片能够缩短SAMP8模型小鼠的潜伏期、明显地增加平台所在象限的时间百分比和进入目标象限的次数,可显著地减少(避暗)错误次数和延长逃避潜伏期;能够下调海马Aβ的表达并上调Neu N蛋白的表达,可显著地减少核糖体的丢失,降低线粒体的肿胀度,改善海马的超微结构。结论通络醒脑泡腾片可通过抑制Aβ沉积,提高Neu N的表达来修复神经元,保护线粒体,从而提高SAMP8小鼠的学习记忆能力。
Objective To observe the effects of Tongluo Xingnao effervescent tablet on Neu N protein and ultrastructure in hippocampus of rapidly aging mouse subline 8 (SAMP8). Methods SAMP8 mice were randomly divided into SAMP8 group, amoxicillin group, Tongluo Xingnao effervescent tablet group (high, middle and low dose), and another anti-fast aging mouse subline 1 (SAMR1) After 60 consecutive days, Morris water maze and avoidance method were used to evaluate the changes of learning and memory. The expression of Aβ and Neu N in hippocampus was detected by immunohistochemistry. The ultrastructure of hippocampus was observed by electron microscopy. Results Tongdu Xingnao effervescent tablet can shorten the incubation period of SAMP8 model mice, significantly increase the time percentage of the platform where the quadrant is located and the number of times it enters the target quadrant, can significantly reduce (avoid dark) the number of errors and extend the escape latency; The expression of hippocampal Aβ and upregulation of Neu N protein can significantly reduce the loss of ribosome, reduce mitochondrial swelling and improve the ultrastructure of the hippocampus. Conclusion Tongluo Xingnao effervescent tablet can repair neurons, protect mitochondria and inhibit learning and memory of SAMP8 mice by inhibiting Aβ deposition and increasing Neu N expression.