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Age-related osteoporosis is associated with the reduced capacity of bone marrow mesenchymal stem cells(BMSCs) to differentiate into osteoblasts instead of adipocytes. However, the molecular mechanisms that decide the fate of BMSCs remain unclear. In our study, micro RNA-23 a, and micro RNA-23b(mi R-23a/b) were found to be markedly downregulated in BMSCs of aged mice and humans. The overexpression of mi R-23a/b in BMSCs promoted osteogenic differentiation, whereas the inhibition of mi R-23a/b increased adipogenic differentiation. Transmembrane protein 64(Tmem64), which has expression levels inversely related to those of mi R-23a/b in aged and young mice, was identified as a major target of mi R-23a/b during BMSC differentiation. In conclusion, our study suggests that mi R-23a/b has a critical role in the regulation of mesenchymal lineage differentiation through the suppression of Tmem64.
Age-related osteoporosis is associated with the reduced capacity of bone marrow mesenchymal stem cells (BMSCs) to differentiate into osteoblasts instead of adipocytes. However, the molecular mechanisms that decide the fate of BMSCs remain unclear. In our study, micro RNA-23 a , and micro RNA-23b (mi R-23a / b) were found to be markedly downregulated in BMSCs of aged mice and humans. The overexpression of mi R-23a / b in BMSCs promoted osteogenic differentiation, 23a / b increased adipogenic differentiation. Transmembrane protein 64 (Tmem64), which has expression levels inversely related to those of mi R-23a / b in aged and young mice, was identified as a major target of mi R-23a / b during BMSC In conclusion, our study suggests that mi R-23a / b has a critical role in the regulation of mesenchymal lineage differentiation through the suppression of Tmem64.