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目的研究两种抗菌剂三氯生(triclosan,TCS)和三氯卡班(triclocarban,TCC)在环境相关浓度水平对嗜热四膜虫体内抗氧化酶活力的影响。方法将处于对数生长期的嗜热四膜虫分别暴露于0(对照)、0.1、100μg/L的TCS和TCC培养24 h,测定嗜热四膜虫体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽-S转移酶(GST)和乳酸脱氢酶(LDH)的活力。结果与对照组比较,各浓度TCS和TCC暴露均可诱导嗜热四膜虫体内SOD活力明显升高(P<0.05或P<0.01),LDH活力明显下降(P<0.01);各浓度TCS暴露均可诱导嗜热四膜虫体内CAT和GST活力均明显升高(P<0.05或P<0.01),但各浓度TCC暴露对这两种酶的影响均不显著。结论 TCS和TCC在环境相关浓度水平下能够诱导嗜热四膜虫产生抗氧化应激。
Objective To study the effects of triclosan (TCS) and triclocarban (TCC), an antimicrobial agent, on the antioxidant enzyme activities of Tetrahymena thermophila in environmental conditions. Methods Tetrahymena thermophila in logarithmic growth phase were exposed to 0 (control), 0.1 and 100 μg / L TCS and TCC for 24 h respectively. The levels of superoxide dismutase (SOD) Catalase (CAT), glutathione-S transferase (GST) and lactate dehydrogenase (LDH) activity. Results Compared with the control group, the concentrations of TCS and TCC could induce the increase of SOD activity (P <0.05 or P <0.01) and LDH activity of Tetrahymena thermophila (P <0.01) (P <0.05 or P <0.01). However, the effects of TCC exposure on these two enzymes were not significant. Conclusion TCS and TCC can induce anti-oxidative stress induced by Tetrahymena thermophila under environment-related concentrations.