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目的:采用抗SP3111抗体Ab2438封闭后的体外受精试验,研究SP3111蛋白在受精及早期胚胎发育过程中的作用。方法:首先在体外受精前,将精子分为3组:实验组、空白对照组、阴性对照组。实验组精子与Ab2438共同孵育1h后进行体外受精,观察2、4、6、8、22h的受精率及碎裂胚胎的发生率。接着将Ab2438作用于受精后的卵母细胞,观察作用22h后的受精率及碎裂胚胎的发生率。结果:抗体Ab2438作用小鼠精子后,碎裂胚胎发生率在加入精子后2、4、6、8、22h分别为5.26%、8.77%、23.25%、43.42%、59.21%,与各对照组碎裂胚胎形成率均有显著性差异(P<0.01)。Ab2438作用于受精后的卵母细胞,22h后实验组的正常胚胎及碎裂胚胎的形成率分别为23.64%和63.64%,与各对照组相比差异有显著性(P<0.01)。结论:抗SP3111抗体对小鼠受精及早期胚胎发育有明显的影响,因此SP3111蛋白可能是一个信号分子,与其他蛋白一起在受精和早期胚胎发育中发挥作用,对SP3111蛋白生殖功能的进一步研究将为不育的分子机制的研究提供新的思路。
OBJECTIVE: To study the effect of SP3111 protein in the process of fertilization and early embryo development by in vitro fertilization test after anti-SP3111 antibody Ab2438 was blocked. Methods: Before in vitro fertilization, sperm were divided into three groups: experimental group, blank control group and negative control group. The experimental group sperm incubated with Ab2438 1h after in vitro fertilization, observed 2,4,6,8,22h fertilization rate and the incidence of fragmented embryos. Ab2438 was then used to fertilize the oocytes and the fertilization rate and the incidence of fragmented embryos were observed after 22 hours. Results: After the sperm was stimulated with Ab2438 antibody, the incidence of fragmented embryos was 5.26%, 8.77%, 23.25%, 43.42% and 59.21% respectively at 4, 6, 8 and 22 h after sperm were added, The rate of formation of split embryos was significantly different (P <0.01). Ab2438 oocytes after fertilization, the formation rate of normal embryos and fragmented embryos was 23.64% and 63.64% respectively in the experimental group after 22h, which was significantly different from that of the control group (P <0.01). CONCLUSIONS: Anti-SP3111 antibody has a significant effect on fertilization and early embryo development in mice. Therefore SP3111 protein may be a signaling molecule that plays a role in fertilization and early embryogenesis together with other proteins. Further study on the reproductive function of SP3111 protein The molecular mechanism of infertility provides a new way of thinking.